Chien Sheng Chen

TL;DR: It is demonstrated that chronic alcohol administration increases mycobiota populations and translocation of fungal &bgr;-glucan into systemic circulation in mice and Manipulating the intestinalMycobiome might be an effective strategy for attenuating alcohol-related liver disease.

TL;DR: In this unit, advances in protein microarray technologies are reviewed, and then a series of examples are presented to illustrate the applications of analytical and functional protein microarrays in both basic and clinical research.

TL;DR: Analysis of RcsBAc and variant RcsB proteins carrying substitutions at Lys180 provided biochemical and physiological evidence implicating Lys180 as a critical residue for RCSB DNA-binding activity, further the likelihood that reversible N ε-Lys acetylation of transcription factors is a mode of regulation of gene expression used by all cells.

TL;DR: A stable superhydrophilic zwitterionic interface on polydimethylsiloxane (PDMS) elastomer by covalent silanization of sulfobetaine silane (SBSi) to resist nonspecific adsorption of bacteria, proteins, and lipids is developed and can be applied to other silicone-based medical devices in a facile way.

TL;DR: A high-throughput protein purification protocol was developed that allowed us to purify 4,256 proteins encoded by the Escherichia coli K12 strain within 10 h, and these proteins were spotted onto glass slides to create E. coli proteome chips, which have the potential to reveal important interactions between proteins and nucleic acids that are time-consuming and difficult to detect using other techniques.