C
Christian Johannes Gloeckner
Researcher at German Center for Neurodegenerative Diseases
Publications - 72
Citations - 4229
Christian Johannes Gloeckner is an academic researcher from German Center for Neurodegenerative Diseases. The author has contributed to research in topics: LRRK2 & Kinase activity. The author has an hindex of 30, co-authored 71 publications receiving 3745 citations. Previous affiliations of Christian Johannes Gloeckner include University of Tübingen & Helmholtz Zentrum München.
Papers
More filters
Journal ArticleDOI
The Parkinson disease causing LRRK2 mutation I2020T is associated with increased kinase activity
Christian Johannes Gloeckner,Norbert Kinkl,Annette Schumacher,Ralf J. Braun,Eric O'Neill,Thomas Meitinger,Walter Kolch,Holger Prokisch,Marius Ueffing +8 more
TL;DR: The disease-associated I2020T mutant shows a significant increase in autophosphorylation of approximately 40% in comparison to wild-type protein in vitro, which suggests that the pathology of PD caused by the I 2020T mutation is associated with an increase rather than a loss in LRRK2 kinase activity.
Journal ArticleDOI
A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover.
Egon Deyaert,Lina Wauters,Lina Wauters,Giambattista Guaitoli,Albert Konijnenberg,Margaux Leemans,Susanne Terheyden,Arsen Petrovic,Rodrigo Gallardo,Laura M Nederveen-Schippers,Panagiotis S Athanasopoulos,Henderikus Pots,Peter J.M. van Haastert,Frank Sobott,Frank Sobott,Christian Johannes Gloeckner,Rouslan G. Efremov,Arjan Kortholt,Wim Versées +18 more
TL;DR: It is shown that oligomerization of a homologous bacterial Roco protein depends on the nucleotide load, which provides insights into the conformational cycle of Roco proteins and suggests a link between oligomersization and disease-associated mutations in LRRK2.
Journal ArticleDOI
LRRK2 controls synaptic vesicle storage and mobilization within the recycling pool.
Giovanni Piccoli,Steven B. Condliffe,Matthias Bauer,Florian Giesert,Karsten Boldt,Silvia De Astis,Andrea Meixner,Hakan Sarioglu,Daniela Vogt-Weisenhorn,Wolfgang Wurst,Christian Johannes Gloeckner,Michela Matteoli,Carlo Sala,Marius Ueffing +13 more
TL;DR: The results suggest that LRRK2 modulates synaptic vesicles trafficking and distribution in neurons and in consequence participates in regulating the dynamics between vesicle pools inside the presynaptic bouton.
Journal ArticleDOI
A novel tandem affinity purification strategy for the efficient isolation and characterisation of native protein complexes.
TL;DR: The power of this novel method is demonstrated by the purification of Raf associated protein complexes from HEK293 cells and subsequent analysis of their protein interaction network by dissection of interaction patterns from the Raf binding partners MEK1 and 14‐3‐3.
Journal ArticleDOI
An organelle-specific protein landscape identifies novel diseases and molecular mechanisms
Karsten Boldt,van Reeuwijk J,Lu Q,Konstantinos Koutroumpas,Nguyen Tm,Yves Texier,Yves Texier,van Beersum Se,Nicola Horn,Willer,Dorus A. Mans,Gerard W. Dougherty,Ideke J.C. Lamers,Karlien L.M. Coene,Heleen H. Arts,Matthew J. Betts,Beyer T,Emine Bolat,Christian Johannes Gloeckner,Haidari K,Lisette Hetterschijt,Daniela Iaconis,Jenkins D,Franziska Klose,Barbara Knapp,Brooke L. Latour,Stef J.F. Letteboer,Carlo Marcelis,Mitic D,Manuela Morleo,Machteld M. Oud,Moniek Riemersma,Suzanne Rix,Paulien A Terhal,Grischa Toedt,van Dam Tj,de Vrieze E,Yasmin Wissinger,Wu Km,Gordana Apic,PL Beales,Oliver E. Blacque,Toby J. Gibson,Martijn A. Huynen,Nicholas Katsanis,Hannie Kremer,Heymut Omran,van Wijk E,Uwe Wolfrum,François Képès,Erica E. Davis,Brunella Franco,Rachel H. Giles,Marius Ueffing,Robert B. Russell,Ronald Roepman +55 more
TL;DR: It is shown that 3M genes are involved in ciliogenesis, and that patient fibroblasts lack cilia, and the landscape suggests other genetic diseases could be ciliary including 3M syndrome.