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Christopher J. Bohlen
Researcher at Genentech
Publications - 7
Citations - 4658
Christopher J. Bohlen is an academic researcher from Genentech. The author has contributed to research in topics: Neurodegeneration & Microglia. The author has an hindex of 5, co-authored 5 publications receiving 2822 citations. Previous affiliations of Christopher J. Bohlen include University of Melbourne.
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Journal ArticleDOI
Neurotoxic reactive astrocytes are induced by activated microglia
Shane A. Liddelow,Kevin A. Guttenplan,Laura E. Clarke,Frederick C. Bennett,Christopher J. Bohlen,Lucas Schirmer,Mariko L. Bennett,Alexandra E. Münch,Won-Suk Chung,Todd C. Peterson,Daniel K. Wilton,Arnaud Frouin,Brooke A. Napier,Nikhil Panicker,Manoj Kumar,Marion S. Buckwalter,David H. Rowitch,Valina L. Dawson,Ted M. Dawson,Beth Stevens,Ben A. Barres +20 more
TL;DR: It is shown that activated microglia induce A1 astrocytes by secreting Il-1α, TNF and C1q, and that these cytokines together are necessary and sufficient to induce A2 astroCytes, which are abundant in various human neurodegenerative diseases.
Journal ArticleDOI
Trem2 restrains the enhancement of tau accumulation and neurodegeneration by β-amyloid pathology
Seung-Hye Lee,William J. Meilandt,Luke Xie,Vineela D. Gandham,Hai Ngu,Kai H. Barck,Mitchell G. Rezzonico,Jose Imperio,Guita Lalehzadeh,Melanie A. Huntley,Kimberly L. Stark,Oded Foreman,Richard A.D. Carano,Brad A. Friedman,Morgan Sheng,Amy Easton,Christopher J. Bohlen,David V. Hansen +17 more
TL;DR: Trem2 deficiency in the pR5-183 mouse model expressing mutant tau alone or in TauPS2APP mice, in which β-amyloid pathology exacerbates tau pathology and neurodegeneration, was examined in this paper.
Journal ArticleDOI
Microglia in Brain Development, Homeostasis, and Neurodegeneration
TL;DR: Recent genetic evidence for microglial involvement in neurodegenerative disease with a focus on Alzheimer disease is summarized, aiming to synthesize varied aspects of microglian biology and highlight microglia as possible targets for therapeutic interventions in neuro degenerative disease.
Journal ArticleDOI
Isolation and Culture of Microglia.
TL;DR: Approaches for purification of microglia by fluorescence‐activated cell sorting using microgla‐specific surface markers and for enrichment of microGlia by magnetic sorting and immunopanning are described.
Journal ArticleDOI
Efficient gene knockout in primary human and murine myeloid cells by non-viral delivery of CRISPR-Cas9
Emily C. Freund,Jaclyn Y. Lock,Jaehak Oh,Timurs Maculins,Lélia Delamarre,Christopher J. Bohlen,Benjamin Haley,Aditya Murthy +7 more
TL;DR: Highly efficient gene editing in primary myeloid cells is made possible by optimized non-viral delivery of CRISPR-Cas9, which enables single and multiplexed gene knockout in monocytes, macrophages, and dendritic cells to advance innate immunity research and cell therapy.