D. Worlitzsch

TL;DR: In CF patients with established lung disease, Pseudomonas aeruginosa was located within hypoxic mucopurulent masses in airway lumens, and in vitro studies revealed that CF-specific increases in epithelial O(2) consumption, linked to increased airway surface liquid (ASL) volume absorption and mucus stasis, generated steep hypoxic gradients within thickened mucus on CF epithelial surfaces prior to infection.

TL;DR: It is concluded that functional lasI and rhlI genes of P. aeruginosa PAO1 play a significant role during lung infection and they might be associated with the production of virulence factors that are controlled by the quorum sensing systems.

TL;DR: In patients with CF, an increase in neutrophilic airway inflammation is found that is positively influenced by rhDNase treatment, and this is the first study to investigate the long-term effect of rh DNase on inflammation in patients with mild lung disease.

TL;DR: Alginate production by PAO1 in murine lungs and by nonmucoid P. aeruginosa strains in patients with CF was reversible after in vitro culture under aerobic conditions, which may contribute to early persistence, leading to chronic P. Aerug inosa infection once stable mucoid strains are generated.

TL;DR: Antibiotic therapy, optimized to target anaerobes in addition to P. aeruginosa, may improve the management of CF lung disease, and cell numbers and antibiotic susceptibilities of facultative and obligate anaerobia were determined.