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Daowen Wang

Researcher at Henan Agricultural University

Publications -  166
Citations -  8567

Daowen Wang is an academic researcher from Henan Agricultural University. The author has contributed to research in topics: Gene & Common wheat. The author has an hindex of 44, co-authored 143 publications receiving 6902 citations. Previous affiliations of Daowen Wang include Chinese Academy of Sciences & John Innes Centre.

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Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion

TL;DR: A CRISPR–Cas9 nickase-cytidine deaminase fusion is used to achieve targeted conversion of cytosine to thymine from position 3 to 9 within the protospacer in both protoplasts and regenerated rice, wheat and maize plants.
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FIT interacts with AtbHLH38 and AtbHLH39 in regulating iron uptake gene expression for iron homeostasis in Arabidopsis.

TL;DR: The data support that ferric-chelate reductase FRO2 and ferrous-transporter IRT1 are the targets of the three transcription factors and the transcription of FRO 2 and I RT1 is directly regulated by a complex of FIT/AtbHLH38 or FIT /Atb HLH39.
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Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice

TL;DR: It is revealed that BE3 and HF1-BE3, but not ABE, induce substantial genome-wide off-target mutations, which are mostly the C→T type of single-nucleotide variants (SNVs) and appear to be enriched in genic regions.