David L Goode

TL;DR: GERP++ is an efficient and effective tool to provide both nucleotide- and element-level constraint scores within deep multiple sequence alignments, and is predicted to predict a higher fraction than earlier estimates largely due to the annotation of longer constrained elements, which improves one to one correspondence between predicted elements with known functional sequences.

TL;DR: This work systematically mutagenized all 41 CR LEE genes and functionally characterized these mutants in vitro and in a murine infection model, identifying 33 virulence factors, including two virulence regulators and a hierarchical switch for type III secretion.

TL;DR: A novel protein, NleA, encoded in a prophage‐associated pathogenicity island within the EHEC genome, distinct from the LEE was determined to play a key role in virulence of C. rodentium in a mouse infection model.

TL;DR: It is found that indel length modulates selection strength, and that indels affecting multiple functionally constrained nucleotides undergo stronger purifying selection than SNPs, and the causal variant underlying some of these associations may be indels.

TL;DR: It is shown that the opossum MHC is gene dense and complex, as in humans, but shares more organizational features with non-mammals.