D
Delai Chen
Researcher at Massachusetts Institute of Technology
Publications - 41
Citations - 6042
Delai Chen is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: In vivo & Microchannel. The author has an hindex of 26, co-authored 41 publications receiving 5445 citations. Previous affiliations of Delai Chen include Harvard University & University of Illinois at Chicago.
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Journal ArticleDOI
Reactions in Droplets in Microfluidic Channels
TL;DR: Fundamental and applied research in chemistry and biology benefits from opportunities provided by droplet-based microfluidic systems, which enable the miniaturization of reactions by compartmentalizing reactions in droplets of femoliter to microliter volumes.
Journal ArticleDOI
Efficiency of siRNA delivery by lipid nanoparticles is limited by endocytic recycling
Gaurav Sahay,William Querbes,Christopher A. Alabi,Ahmed A. Eltoukhy,Sovan Sarkar,Christopher Zurenko,Emmanouil D. Karagiannis,Kevin T. Love,Delai Chen,Roberto Zoncu,Yosef Buganim,Avi Schroeder,Robert Langer,Daniel G. Anderson +13 more
TL;DR: In this paper, the authors examined cellular uptake of siRNA delivered in lipid nanoparticles (LNPs) using cellular trafficking probes in combination with automated high-throughput confocal microscopy as well as defined perturbations of cellular pathways paired with systems biology approaches to uncover protein-protein and protein-small molecule interactions.
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Degradable lipid nanoparticles with predictable in vivo siRNA delivery activity.
Kathryn A. Whitehead,J. Robert Dorkin,Arturo J. Vegas,Philip H. Chang,Omid Veiseh,Jonathan Matthews,Owen S. Fenton,Yunlong Zhang,Karsten Olejnik,Volkan Yesilyurt,Delai Chen,Scott A Barros,Boris Klebanov,Tatiana Novobrantseva,Robert Langer,Daniel G. Anderson +15 more
TL;DR: It is shown that lipidoid nanoparticles mediate potent gene knockdown in hepatocytes and immune cell populations upon IV administration to mice (siRNA EC50 values as low as 0.01 mg/kg); new design criteria that reliably predict in vivo siRNA delivery efficacy without any prior biological testing are described.
Journal ArticleDOI
Rapid Discovery of Potent siRNA-Containing Lipid Nanoparticles Enabled by Controlled Microfluidic Formulation
Delai Chen,Kevin T. Love,Yi Chen,Ahmed A. Eltoukhy,Christian J. Kastrup,Gaurav Sahay,Alvin Jeon,Yizhou Dong,Kathryn A. Whitehead,Daniel G. Anderson,Daniel G. Anderson +10 more
TL;DR: It is shown that gene silencing assays employing lipoplexes result in a high rate of false negatives that can largely be avoided through formulation, which will facilitate the discovery of next-generation reagents for LNP-mediated nucleic acid delivery.
Journal ArticleDOI
Lipopeptide nanoparticles for potent and selective siRNA delivery in rodents and nonhuman primates
Yizhou Dong,Kevin T. Love,Kevin T. Love,J. Robert Dorkin,Sasilada Sirirungruang,Yunlong Zhang,Delai Chen,Delai Chen,Roman L. Bogorad,Hao Yin,Yi Chen,Arturo J. Vegas,Arturo J. Vegas,Christopher A. Alabi,Gaurav Sahay,Karsten Olejnik,Weiheng Wang,Weiheng Wang,Avi Schroeder,Abigail K. R. Lytton-Jean,Daniel J. Siegwart,Akin Akinc,Carmen Barnes,Scott A Barros,Mary Carioto,Kevin Fitzgerald,Julia Hettinger,Varun Kumar,Tatiana Novobrantseva,June Qin,William Querbes,Victor Koteliansky,Robert Langer,Daniel G. Anderson,Daniel G. Anderson +34 more
TL;DR: In this article, the authors report lipopeptide nanoparticles as potent and selective siRNA carriers with a wide therapeutic index for gene silencing in liver parenchymal cells.