Delai Chen

TL;DR: Fundamental and applied research in chemistry and biology benefits from opportunities provided by droplet-based microfluidic systems, which enable the miniaturization of reactions by compartmentalizing reactions in droplets of femoliter to microliter volumes.

TL;DR: In this paper, the authors examined cellular uptake of siRNA delivered in lipid nanoparticles (LNPs) using cellular trafficking probes in combination with automated high-throughput confocal microscopy as well as defined perturbations of cellular pathways paired with systems biology approaches to uncover protein-protein and protein-small molecule interactions.

TL;DR: It is shown that lipidoid nanoparticles mediate potent gene knockdown in hepatocytes and immune cell populations upon IV administration to mice (siRNA EC50 values as low as 0.01 mg/kg); new design criteria that reliably predict in vivo siRNA delivery efficacy without any prior biological testing are described.

TL;DR: It is shown that gene silencing assays employing lipoplexes result in a high rate of false negatives that can largely be avoided through formulation, which will facilitate the discovery of next-generation reagents for LNP-mediated nucleic acid delivery.

TL;DR: In this article, the authors report lipopeptide nanoparticles as potent and selective siRNA carriers with a wide therapeutic index for gene silencing in liver parenchymal cells.