Showing papers by "Donna E. Davies published in 1995"
TL;DR: A difference in activity of the C-terminally extended form of AR may be of biological significance since differential proteolytic processing of the AR precursor in vivo could result in production of multiple forms of the growth factor with differing affinities for the EGF receptor and hence differing biological potencies.
37 citations
TL;DR: It is concluded that the B-loop β-sheet of hTGFα is an important determinant of EGF receptor binding affinity and biological activity.
34 citations
TL;DR: Failure of these agents to block cell growth or influence growth factor-induced mitogenesis indicated that the biochemical pathway(s) by which CyA or FK506 inhibited cell growth did not depend solely on inhibition of calcineurin.
29 citations
TL;DR: This study has measured neutrophil influx and release of interleukin-8 into asthmatic airways after endobronchial challenge with either allergen or saline solution.
Abstract: Neutrophils have been associated with the late asthmatic reaction. However, their role in this disease is not well understood. In this study, we have measured neutrophil influx and release of interleukin-8 into asthmatic airways after endobronchial challenge with either allergen or saline solution.
29 citations
TL;DR: The enantiomers of exo 2-azabicyclo[2,2,1]heptane-3-carboxylic acid have been independently synthesized by the aza Diels Alder reaction using a chiral auxiliary.
15 citations
TL;DR: Although anthralin and hypericin both inhibit EGF signalling, they do not act specifically on the EGF-R pathway, and their mechanisms of action do not appear to be comparable.
14 citations
TL;DR: Two morphologically distinct cell lines, GP2d and GP5d, derived from the same adenocarcinoma of the colon, have been established and characterised, indicating separation of growth and morphological responses to EGF.
Abstract: Two morphologically distinct cell lines, GP2d and GP5d, derived from the same adenocarcinoma of the colon, have been established and characterised. Both clones have the same genetic changes, consistent with the usual pattern of tumour progression in colon cancer. The cells also have an inverted duplication of bands 10q11 to 10q21, but Southern blot analysis failed to identify any translocations involving the ret protooncogene, which maps to this region. GP2d grew by spreading from the edges of microcolonies to form a confluent layer of cells. GP5d grew in discrete islands of cells forming multi-layered colonies. These differing patterns of growth correlated with variation in expression or cellular distribution of alpha 2-integrin, desmoplakin and e-cadherin. Only GP2d responded to exogenously added epidermal growth factor (EGF), transforming growth factor-alpha (TGF alpha) or insulin with an increase in cell numbers, even though both cell lines possessed similar numbers of EGF receptors. Analysis of EGF receptor ligand expression showed that GP5d cells expressed relatively more TGF alpha mRNA than did GP2d; in contrast, amphiregulin mRNA, which was abundant in GP2d, was virtually undetectable in GP5d. Even though GP5d failed to exhibit a growth response to EGF, it underwent a marked epithelial-mesenchymal transition when treated with EGF, indicating separation of growth and morphological responses to EGF.
13 citations
TL;DR: The results indicate that translocation of EGF-R to the nucleus is a controlled process and they suggest that E GF-R may directly influence nuclear function.
13 citations
TL;DR: In this paper, two proline mimetics, the enantiomers of 2-azabicyclo[2,2,1]heptane-3-carboxylic acid, have been incorporated in place of Pro30 into synthetic peptides based on the B-loop β-sheet sequence of human transforming growth factor-α (TGF-α) (residues Cys21-Cys32).
11 citations