Douglas J. Lamont

TL;DR: The results elucidate how PROTAC-induced de novo contacts dictate preferential recruitment of a target protein into a stable and cooperative complex with an E3 ligase for selective degradation.

TL;DR: The data suggest that Keap1 is a specialized sensor that quantifies stress by monitoring the intracellular concentrations of NO, Zn2+, and alkenals, which collectively serve as second messengers that may signify danger and/or damage.

TL;DR: A subset of proteins was identified that exist in pools with different turnover rates depending on their subcellular localization, suggesting a general mechanism whereby their assembly is controlled in a different sub cellular location to their main site of function.

TL;DR: Mass spectrometry-based proteomics is used to provide an unbiased, quantitative, and high throughput approach for measuring the subcellular distribution of the proteome, termed “spatial proteomics” and facilitates a proteome-wide comparison of changes in protein localization in response to a wide range of physiological and experimental perturbations.

TL;DR: Homo-PROTACs is described as an approach to dimerize an E3 ligase to trigger its suicide-type chemical knockdown inside cells and small molecules that can induce the homo-dimerization of E3 ubiquitin ligases and cause their proteasome-dependent degradation.