E
Edze R. Westra
Researcher at University of Exeter
Publications - 89
Citations - 9506
Edze R. Westra is an academic researcher from University of Exeter. The author has contributed to research in topics: CRISPR & Population. The author has an hindex of 30, co-authored 80 publications receiving 7781 citations. Previous affiliations of Edze R. Westra include Wageningen University and Research Centre & McGill University.
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Journal ArticleDOI
Small CRISPR RNAs guide antiviral defense in prokaryotes
Stan J. J. Brouns,Matthijs M. Jore,Magnus Lundgren,Edze R. Westra,Rik J. H. Slijkhuis,Ambrosius P. Snijders,Mark J. Dickman,Kira S. Makarova,Eugene V. Koonin,John van der Oost +9 more
TL;DR: The results demonstrate that the formation of mature guide RNAs by the CRISPR RNA endonuclease subunit of Cascade is a mechanistic requirement for antiviral defense.
Journal ArticleDOI
Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence
Ekaterina Semenova,Matthijs M. Jore,Kirill A. Datsenko,Anna Semenova,Edze R. Westra,Barry L. Wanner,John van der Oost,Stan J. J. Brouns,Konstantin Severinov +8 more
TL;DR: It is proposed that the crRNA seed sequence plays a role in the initial scanning of invader DNA for a match, before base pairing of the full-length spacer occurs, which may enhance the protospacer locating efficiency of the E. coli Cascade complex.
Journal ArticleDOI
Unravelling the structural and mechanistic basis of CRISPR-Cas systems.
TL;DR: This Review summarizes the recent structural and biochemical insights that have been gained for the three major types of CRISPR–Cas systems, which together provide a detailed molecular understanding of the unique and conserved mechanisms of RNA-guided adaptive immunity in bacteria and archaea.
Journal ArticleDOI
Structural basis for CRISPR RNA-guided DNA recognition by Cascade
Matthijs M. Jore,Magnus Lundgren,Magnus Lundgren,Esther van Duijn,Jelle B. Bultema,Edze R. Westra,Sakharam P Waghmare,Blake Wiedenheft,Blake Wiedenheft,Ümit Pul,Reinhild Wurm,Rolf Wagner,Marieke R Beijer,Arjan Barendregt,Kaihong Zhou,Kaihong Zhou,Ambrosius P. Snijders,Ambrosius P. Snijders,Mark J. Dickman,Jennifer A. Doudna,Jennifer A. Doudna,Egbert J. Boekema,Albert J. R. Heck,John van der Oost,Stan J. J. Brouns +24 more
TL;DR: The composition and low-resolution structure of Cascade is presented and it is shown how it recognizes double-stranded DNA targets in a sequence-specific manner and suggests that continuous invader DNA surveillance takes place without energy investment.
Journal ArticleDOI
CRISPR-based adaptive and heritable immunity in prokaryotes.
TL;DR: Analysis of key Cas proteins indicate that, despite some functional analogies, this fascinating prokaryotic system shares no phylogenetic relation with the eukaryotic RNA interference system.