R
Reinhild Wurm
Researcher at University of Düsseldorf
Publications - 19
Citations - 2025
Reinhild Wurm is an academic researcher from University of Düsseldorf. The author has contributed to research in topics: Transcription (biology) & CRISPR. The author has an hindex of 17, co-authored 19 publications receiving 1861 citations.
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Journal ArticleDOI
Structural basis for CRISPR RNA-guided DNA recognition by Cascade
Matthijs M. Jore,Magnus Lundgren,Magnus Lundgren,Esther van Duijn,Jelle B. Bultema,Edze R. Westra,Sakharam P Waghmare,Blake Wiedenheft,Blake Wiedenheft,Ümit Pul,Reinhild Wurm,Rolf Wagner,Marieke R Beijer,Arjan Barendregt,Kaihong Zhou,Kaihong Zhou,Ambrosius P. Snijders,Ambrosius P. Snijders,Mark J. Dickman,Jennifer A. Doudna,Jennifer A. Doudna,Egbert J. Boekema,Albert J. R. Heck,John van der Oost,Stan J. J. Brouns +24 more
TL;DR: The composition and low-resolution structure of Cascade is presented and it is shown how it recognizes double-stranded DNA targets in a sequence-specific manner and suggests that continuous invader DNA surveillance takes place without energy investment.
Journal ArticleDOI
Identification and characterization of E. coli CRISPR-cas promoters and their silencing by H-NS.
TL;DR: It is demonstrated that the DNA‐binding protein H‐NS is involved in silencing the CRISPR‐cas promoters, resulting in cryptic Cas protein expression, and suggested a potential link between two prokaryotic defence systems against foreign DNA.
Journal ArticleDOI
H-NS-mediated repression of CRISPR-based immunity in Escherichia coli K12 can be relieved by the transcription activator LeuO
Edze R. Westra,Ümit Pul,Nadja Heidrich,Matthijs M. Jore,Magnus Lundgren,Thomas Stratmann,Reinhild Wurm,Amanda Raine,Melina Mescher,Luc van Heereveld,Marieke Mastop,E. Gerhart H. Wagner,Karin Schnetz,John van der Oost,Rolf Wagner,Stan J. J. Brouns +15 more
TL;DR: It is demonstrated that in E. coli H‐NS and LeuO are antagonistic regulators of CRISPR‐based immunity, which leads to enhanced protection against phage infection.
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Structural basis for H-NS-mediated trapping of RNA polymerase in the open initiation complex at the rrnB P1
TL;DR: A model is presented for the specific repression of transcription initation at the rrnB P1 by H-NS, which stimulates open complex formation by forming a ternary RNAP·H-NS·DNA complex, while simultaneously stabilizing it to such an extent that promoter clearance cannot occur.
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The Streptomyces coelicolor GlnR regulon: identification of new GlnR targets and evidence for a central role of GlnR in nitrogen metabolism in actinomycetes
TL;DR: It is suggested that GlnR‐mediated regulation is not restricted to Streptomyces but constitutes a regulon conserved in many actinomycetes and is able to interact with these glnA upstream regions.