E
Elsayed Tharwat Tolba Mohamed
Researcher at Technical University of Denmark
Publications - 13
Citations - 315
Elsayed Tharwat Tolba Mohamed is an academic researcher from Technical University of Denmark. The author has contributed to research in topics: Fermentation & Commodity chemicals. The author has an hindex of 7, co-authored 11 publications receiving 168 citations. Previous affiliations of Elsayed Tharwat Tolba Mohamed include Novo Nordisk Foundation.
Papers
More filters
Journal ArticleDOI
Adaptive laboratory evolution of Pseudomonas putida KT2440 improves p-coumaric and ferulic acid catabolism and tolerance
Elsayed Tharwat Tolba Mohamed,Allison Z. Werner,Allison Z. Werner,Davinia Salvachúa,Christine A. Singer,Kiki Szostkiewicz,Manuel Rafael Jiménez-Díaz,Manuel Rafael Jiménez-Díaz,Thomas Eng,Thomas Eng,Mohammad S. Radi,Blake A. Simmons,Blake A. Simmons,Aindrila Mukhopadhyay,Aindrila Mukhopadhyay,Markus J. Herrgård,Steven W. Singer,Steven W. Singer,Gregg T. Beckham,Gregg T. Beckham,Adam M. Feist,Adam M. Feist,Adam M. Feist +22 more
TL;DR: This work demonstrates increased tolerance towards and growth rate at the expense of hydroxycinnamic acids and presents new targets for improving P. putida for microbial lignin valorization.
Journal ArticleDOI
Adaptive laboratory evolution of tolerance to dicarboxylic acids in Saccharomyces cerevisiae.
Rui Pedro Gomes Pereira,Yongjun Wei,Elsayed Tharwat Tolba Mohamed,Mohammad S. Radi,Carl Malina,Markus J. Herrgård,Adam M. Feist,Jens Nielsen,Yun Chen +8 more
TL;DR: Adaptive laboratory evolution was used to explore the tolerance mechanisms that Saccharomyces cerevisiae can evolve in the presence of inhibiting concentrations of three dicarboxylic acids, and the amplification of QDR3 was shown to confer tolerance towards muconic acid and glutaconic acid.
Journal ArticleDOI
Generation of a platform strain for ionic liquid tolerance using adaptive laboratory evolution
Elsayed Tharwat Tolba Mohamed,Shizeng Wang,Shizeng Wang,Shizeng Wang,Rebecca M. Lennen,Markus J. Herrgård,Blake A. Simmons,Blake A. Simmons,Steven W. Singer,Steven W. Singer,Adam M. Feist,Adam M. Feist +11 more
TL;DR: The generated strains represent the best performing platform strains available for bioproduction utilizing IL-treated renewable substrates, and the TALE method was highly successful in overcoming the general issue of substrate toxicity and has great promise for use in tolerance engineering.
Journal ArticleDOI
Laboratory evolution reveals regulatory and metabolic trade-offs of glycerol utilization in Saccharomyces cerevisiae
Tomas Strucko,Katharina Zirngibl,Filipa Pereira,Eleni Kafkia,Elsayed Tharwat Tolba Mohamed,Mandy Rettel,Frank Stein,Adam M. Feist,Paula Jouhten,Kiran Raosaheb Patil,Jochen Förster +10 more
TL;DR: These findings not only have a direct relevance for improving glycerol-based bioprocesses, but also illustrate how a metabolic pathway can remain unexploited due to fitness trade-offs in other, ecologically important, traits.
Journal ArticleDOI
Elucidating aromatic acid tolerance at low pH in Saccharomyces cerevisiae using adaptive laboratory evolution
Rui Pedro Gomes Pereira,Elsayed Tharwat Tolba Mohamed,Mohammad S. Radi,Markus J. Herrgård,Adam M. Feist,Adam M. Feist,Jens Nielsen,Yun Chen +7 more
TL;DR: It is shown that regulating the activity of transporters is a major mechanism to improve tolerance to aromatic acids and can be used to modulate the intracellular concentration of aromatic compounds to optimize the excretion of such products while keeping precursor molecules inside the cell.