Adaptive laboratory evolution of Pseudomonas putida KT2440 improves p-coumaric and ferulic acid catabolism and tolerance
Elsayed Tharwat Tolba Mohamed,Allison Z. Werner,Allison Z. Werner,Davinia Salvachúa,Christine A. Singer,Kiki Szostkiewicz,Manuel Rafael Jiménez-Díaz,Manuel Rafael Jiménez-Díaz,Thomas Eng,Thomas Eng,Mohammad S. Radi,Blake A. Simmons,Blake A. Simmons,Aindrila Mukhopadhyay,Aindrila Mukhopadhyay,Markus J. Herrgård,Steven W. Singer,Steven W. Singer,Gregg T. Beckham,Gregg T. Beckham,Adam M. Feist,Adam M. Feist,Adam M. Feist +22 more
Reads0
Chats0
TLDR
This work demonstrates increased tolerance towards and growth rate at the expense of hydroxycinnamic acids and presents new targets for improving P. putida for microbial lignin valorization.About:
This article is published in Metabolic Engineering Communications.The article was published on 2020-08-29 and is currently open access. It has received 68 citations till now. The article focuses on the topics: Pseudomonas putida & Ferulic acid.read more
Citations
More filters
Journal ArticleDOI
Lignin valorization by bacterial genus Pseudomonas: State-of-the-art review and prospects.
Manish Kumar,Siming You,Jingzi Beiyuan,Gang Luo,Juhi Gupta,Sunil Kumar,Lal Singh,Shicheng Zhang,Daniel C.W. Tsang +8 more
TL;DR: The potential of genus Pseudomonas in lignin valorization is critically reviewed along with the advanced genetic techniques and tools to ease the use of lign in/lignin-model compounds for the synthesis of bioproducts.
Journal ArticleDOI
Defined Microbial Mixed Culture for Utilization of Polyurethane Monomers
Romualdus Nugraha Catur Utomo,Wing-Jin Li,Till Tiso,Christian Eberlein,Moritz Doeker,Hermann J. Heipieper,Andreas Jupke,Nick Wierckx,Nick Wierckx,Lars M. Blank +9 more
TL;DR: In this article, a biotechnological route to utilize PU hydrolysate as a carbon source for a defined microbial mixed culture was proposed, which consists of dedicated microbes, each trained to utilize a single PU monomer and further engineered to produce valuable products.
Journal ArticleDOI
Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440.
Allison Z. Werner,Rita Clare,Mand Thomas David,Isabel Pardo,Kelsey J. Ramirez,Stefan J. Haugen,Felicia Bratti,Gara N. Dexter,Joshua R. Elmore,Jay D. Huenemann,George L. Peabody,Christopher W. Johnson,Nicholas A. Rorrer,Davinia Salvachúa,Adam M. Guss,Gregg T. Beckham +15 more
TL;DR: In this paper, the authors performed four sequential metabolic engineering efforts in Pseudomonas putida KT2440 to enable the conversion of PET glycolysis products via: (i) ethylene glycol utilization by constitutive expression of native genes, (ii) terephthalate (TPA) catabolism by expression of tphA2IIA3IIBIIA1II from Comamonas and tpaK from Rhodococcus jostii, (iii) bis(2-hydroxyethyl) TPA hydrolysis to TPA
Journal ArticleDOI
The metabolic potential of plastics as biotechnological carbon sources – Review and targets for the future
TL;DR: In this article , the authors summarized well-known monomer degradation pathways and computed their theoretical yields for industrially interesting products, and calculated replacement scenarios of existing fossil-based synthesis routes for the same products.
Journal ArticleDOI
Towards robust Pseudomonas cell factories to harbour novel biosynthetic pathways.
Nora Lisa Bitzenhofer,Luzie Kruse,Stephan Thies,Benedikt Wynands,Thorsten Lechtenberg,Jakob Rönitz,Ekaterina Kozaeva,Nicolas Wirth,Christian Eberlein,Karl-Erich Jaeger,Pablo I. Nikel,Hermann J. Heipieper,Nick Wierckx,Anita Loeschcke +13 more
TL;DR: In this paper, the authors discuss major resistance strategies of Pseudomonads along with approaches pursued for their targeted exploitation and engineering in a biotechnological context and highlight strategies for the identification of yet unknown tolerance-associated genes and their utilisation for engineering next-generation chassis and finally discuss effective measures for pathway fine-tuning to establish stable cell factories for the effective production of natural compounds and novel biochemicals.
References
More filters
Journal ArticleDOI
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
Journal ArticleDOI
Enzymatic assembly of DNA molecules up to several hundred kilobases
Daniel G. Gibson,Lei Young,Ray-Yuan Chuang,J. Craig Venter,Clyde A. Hutchison,Hamilton O. Smith +5 more
TL;DR: An isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5′ exonuclease, a DNA polymerase and a DNA ligase is described.
Journal ArticleDOI
The Catalytic Valorization of Lignin for the Production of Renewable Chemicals
TL;DR: Biomass is an important feedstock for the renewable production of fuels, chemicals, and energy, and it recently surpassed hydroelectric energy as the largest domestic source of renewable energy.
Journal ArticleDOI
Lignin valorization: improving lignin processing in the biorefinery.
Arthur J. Ragauskas,Gregg T. Beckham,Mary J. Biddy,Richard P. Chandra,Fang Chen,Mark F. Davis,Brian H. Davison,Richard A. Dixon,Paul Gilna,Martin Keller,Paul Langan,Amit K. Naskar,John N. Saddler,Timothy J. Tschaplinski,Gerald A. Tuskan,Charles E. Wyman +15 more
TL;DR: Recent developments in genetic engineering, enhanced extraction methods, and a deeper understanding of the structure of lignin are yielding promising opportunities for efficient conversion of this renewable resource to carbon fibers, polymers, commodity chemicals, and fuels.
Journal ArticleDOI
Industrial biocatalysis today and tomorrow
TL;DR: Biocatalytic processes can now be carried out in organic solvents as well as aqueous environments, so that apolar organic compounds aswell as water-soluble compounds can be modified selectively and efficiently with enzymes and bioc atalytically active cells.
Related Papers (5)
Pseudomonas putida as a functional chassis for industrial biocatalysis: From native biochemistry to trans-metabolism.
Pablo I. Nikel,Víctor de Lorenzo +1 more
A field of dreams: Lignin valorization into chemicals, materials, fuels, and health-care products
Judith Becker,Christoph Wittmann +1 more