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Erbay Yigit
Researcher at New England Biolabs
Publications - 20
Citations - 1817
Erbay Yigit is an academic researcher from New England Biolabs. The author has contributed to research in topics: RNA & RNase P. The author has an hindex of 10, co-authored 18 publications receiving 1675 citations. Previous affiliations of Erbay Yigit include University of Massachusetts Medical School & Northwestern University.
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Journal ArticleDOI
Analysis of the C. elegans Argonaute Family Reveals that Distinct Argonautes Act Sequentially during RNAi
Erbay Yigit,Pedro J. Batista,Yanxia Bei,Ka Ming Pang,Chun-Chieh G. Chen,Niraj H. Tolia,Leemor Joshua-Tor,Shohei Mitani,Martin J. Simard,Craig C. Mello +9 more
TL;DR: Analysis of single- and multiple-AGO mutant strains reveals functions in several pathways, including chromosome segregation, fertility, and at least two separate steps in the RNAi pathway, supporting a two-step model for RNAi.
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The dsRNA Binding Protein RDE-4 Interacts with RDE-1, DCR-1, and a DExH-Box Helicase to Direct RNAi in C. elegans
TL;DR: This work shows that the C. elegans RNAi pathway gene, rde-4, encodes a dsRNA binding protein that interacts during RNAi with RNA identical to the trigger dsRNAs, and suggests a model in which RDE-4 and Rde-1 function together to detect and retain foreign ds RNA and to present this ds RNAs to DCR-1 for processing.
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A method for selectively enriching microbial DNA from contaminating vertebrate host DNA
George R. Feehery,Erbay Yigit,Samuel O. Oyola,Bradley W. Langhorst,Victor T. Schmidt,Victor T. Schmidt,Fiona J. Stewart,Eileen T. Dimalanta,Linda A. Amaral-Zettler,Linda A. Amaral-Zettler,Theodore B. Davis,Michael A. Quail,Sriharsa Pradhan +12 more
TL;DR: This study used a methyl-CpG binding domain (MBD) to separate methylated host DNA from microbial DNA based on differences in CpG methylation density, and found that enrichment using the MBD-Fc method holds promise for targeted microbiome sequence analysis across a broad range of sample types.
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A novel enrichment strategy reveals unprecedented number of novel transcription start sites at single base resolution in a model prokaryote and the gut microbiome
TL;DR: Cappable-seq allows for the first time the capture of the 5′ end of primary transcripts, which enables a unique robust TSS determination in bacteria and microbiomes.
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An RIG-I-Like RNA helicase mediates antiviral RNAi downstream of viral siRNA biogenesis in Caenorhabditis elegans.
TL;DR: This work developed a transgenic C. elegans strain that expressed intense green fluorescence from a chromosomally integrated flock house virus replicon only after knockdown or knockout of a gene required for antiviral RNAi, demonstrating both conserved and unique strategies of C. nematodes in antiviral defense.