C
Craig C. Mello
Researcher at University of Massachusetts Medical School
Publications - 130
Citations - 42718
Craig C. Mello is an academic researcher from University of Massachusetts Medical School. The author has contributed to research in topics: RNA interference & Argonaute. The author has an hindex of 63, co-authored 126 publications receiving 39941 citations. Previous affiliations of Craig C. Mello include Harvard University & University of Massachusetts Amherst.
Papers
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Journal ArticleDOI
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Andrew Fire,SiQun Xu,Mary K. Montgomery,Steven A. Kostas,Steven A. Kostas,Samuel E. Driver,Craig C. Mello +6 more
TL;DR: To their surprise, it was found that double-stranded RNA was substantially more effective at producing interference than was either strand individually, arguing against stochiometric interference with endogenous mRNA and suggesting that there could be a catalytic or amplification component in the interference process.
Journal ArticleDOI
Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences.
TL;DR: A dominant behavioral marker, rol‐6(su‐1006), and an efficient microinjection procedure which facilitate the recovery of Caenorhabditis elegans transformants are described and it is shown that low copy number extrachromosomal transformation can be achieved by adjusting the relative concentration of DNA molecules in the injection mixture.
Journal ArticleDOI
Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing
Alla Grishok,Amy E. Pasquinelli,Darryl Conte,Na Li,Susan Parrish,Ilho Ha,David L. Baillie,Andrew Fire,Gary Ruvkun,Craig C. Mello +9 more
TL;DR: It is shown that inactivation of genes related to RNAi pathway genes, a homolog of Drosophila Dicer (dcr-1), and two homologs of rde-1 (alg-1 and alg-2), cause heterochronic phenotypes similar to lin-4 and let-7 mutations.
Patent
Genetic Inhibition by Double-Stranded RNA
Andrew Fire,Stephen A. Kostas,Mary K. Montgomery,Lisa Timmons,SiQun Xu,Hiroaki Tabara,Samuel E. Driver,Craig C. Mello +7 more
TL;DR: In this article, a double-stranded RNA has been used to inhibit gene expression of a target gene in a living cell in order to identify the source and target genes in the cell.
Journal ArticleDOI
The rde-1 Gene, RNA Interference, and Transposon Silencing in C. elegans
Hiroaki Tabara,Madathia Sarkissian,William G. Kelly,Jamie Fleenor,Alla Grishok,Lisa Timmons,Andrew Fire,Craig C. Mello +7 more
TL;DR: It is shown that rde-1 is a member of the piwi/sting/argonaute/zwille/eIF2C gene family conserved from plants to vertebrates and the possibility that one natural function of RNAi is transposon silencing is discussed.