Estienne C. Swart

TL;DR: With more chromosomes than any other sequenced genome, the macronuclear genome of Oxytricha trifallax has a unique and complex architecture, including alternative fragmentation and predominantly single-gene chromosomes.

TL;DR: The Oxytricha germline genome is reported and compared to the somatic genome to present a global view of its massive scale of genome rearrangements and provide a draft of a scrambled genome and a powerful model for studies of genome reshaping.

TL;DR: Evidence is provided, based on ribosomal profiling and “stop” codon depletion shortly before coding sequence ends, that mRNA 3′ ends may contribute to distinguishing stop from sense in a context- dependent manner and it is proposed that such context-dependent termination/readthrough suppression near transcript ends enables genetic code evolution.

TL;DR: It is shown that germline-limited transposase genes play key roles in this process of genome-wide DNA excision, which suggests that transposases function in large eukaryotic genomes containing thousands of active transposons.

TL;DR: The sequencing of the Stentor coeruleus macronuclear genome is reported and key features of the genome are revealed, suggesting that ciliate-specific genetic codes arose after StENTor branched from other ciliates and that ploidy correlates with StentOr's cell size.