G
G. Guy Bushkin
Researcher at Massachusetts Institute of Technology
Publications - 13
Citations - 1304
G. Guy Bushkin is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: CRISPR & Cas9. The author has an hindex of 8, co-authored 12 publications receiving 975 citations. Previous affiliations of G. Guy Bushkin include Boston University.
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Journal ArticleDOI
Perturbation of m6A Writers Reveals Two Distinct Classes of mRNA Methylation at Internal and 5′ Sites
Schraga Schwartz,Maxwell R. Mumbach,Marko Jovanovic,Timothy C. Wang,Timothy C. Wang,Karolina Maciag,Karolina Maciag,G. Guy Bushkin,Philipp Mertins,Dmitry Ter-Ovanesyan,Naomi Habib,Davide Cacchiarelli,Davide Cacchiarelli,Neville E. Sanjana,Elizaveta Freinkman,Michael E. Pacold,Michael E. Pacold,Rahul Satija,Tarjei S. Mikkelsen,Tarjei S. Mikkelsen,Nir Hacohen,Nir Hacohen,Feng Zhang,Feng Zhang,Feng Zhang,Steven A. Carr,Eric S. Lander,Eric S. Lander,Eric S. Lander,Aviv Regev,Aviv Regev,Aviv Regev +31 more
TL;DR: A dense network of proteins interacting with METTL3, a component of the methyltransferase complex, is identified, and it is shown that three of them (WTAP, METTL14, and KIAA1429) are required for methylation.
Journal ArticleDOI
Suggestive evidence for Darwinian selection against asparagine-linked glycans of Plasmodium falciparum and Toxoplasma gondii
G. Guy Bushkin,Daniel M. Ratner,Jike Cui,Sulagna Banerjee,Manoj T. Duraisingh,Cameron V. Jennings,Jeffrey D. Dvorin,Marc-Jan Gubbels,Seth D. Robertson,Martin Steffen,Barry R. O'Keefe,Phillips W. Robbins,John Samuelson +12 more
TL;DR: It is concluded that possible selection against N-glycans in protists with apicoplasts occurs by eliminating N- Glycans (Theileria), reducing their length (Plasmodium), or reducing the number of N- glycan sites (Toxoplasma).
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Strategies To Discover the Structural Components of Cyst and Oocyst Walls
TL;DR: The structural components of cyst and oocyst walls are discovered based upon a few simple assumptions and biochemical methods to dissect fungal walls work well, although the results are often unexpected.
Journal ArticleDOI
New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi.
Valmik K. Vyas,G. Guy Bushkin,Douglas A. Bernstein,Douglas A. Bernstein,Matthew A. Getz,Matthew A. Getz,Magdalena Sewastianik,M. Inmaculada Barrasa,David P. Bartel,David P. Bartel,Gerald R. Fink +10 more
TL;DR: In this paper, the authors present new vectors for clustered regularly interspaced short palindromic repeat (CRISPR) mutagenesis in Candida albicans, Saccharomyces cerevisiae, Candida glabrata, and Naumovozyma castellii.
Journal ArticleDOI
Evidence for a Structural Role for Acid-Fast Lipids in Oocyst Walls of Cryptosporidium, Toxoplasma, and Eimeria
G. Guy Bushkin,Edwin Motari,Andrea Carpentieri,Jitender P. Dubey,Catherine E. Costello,Phillips W. Robbins,John Samuelson +6 more
TL;DR: All oocyst walls are acid fast, have a rigid bilayer, dissolve in organic solvents, and contain a complex set of triglycerides rich in polyhydroxy and long fatty acyl chains that might be synthesized by an abundant polyketide synthase.