Graciela Fuertes

TL;DR: In this article, the subcellular distribution of the different forms of proteasomes using subunit specific antibodies is investigated, and it is shown that different regulatory complexes and subpopulations have different distributions within mammalian cells and, therefore, that the distribution is more complex than has been reported for yeast proteasome.

TL;DR: Findings indicate partially overlapping functions for LAMP-1 and -2 in lysosome biogenesis, autophagy, and cholesterol homeostasis as well as reduced amounts of lipid droplets in double deficient mice.

TL;DR: Although there appear not to be differences in proteasome composition in the alpha subunits (C8 and C9) in the different locations, the relative amounts of some beta subunits varied, which probably reflect differences in the function of proteasomes in distinct cell compartments.

TL;DR: The results show that the activity of the various proteolytic pathways depends on the growth conditions of cells and will be useful for investigation of the specific signals that control their activity.

TL;DR: It is concluded that, although proteasomes are responsible of about 40-60% of the degradation of short-lived proteins in normal human fibroblasts, lysosomes have also an important participation in the degradation with a half-life of approximately 1h.