G
Gwynedd A. Benders
Researcher at J. Craig Venter Institute
Publications - 11
Citations - 4474
Gwynedd A. Benders is an academic researcher from J. Craig Venter Institute. The author has contributed to research in topics: Genome & Gene. The author has an hindex of 11, co-authored 11 publications receiving 4076 citations. Previous affiliations of Gwynedd A. Benders include Torrey Pines Institute for Molecular Studies.
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Journal ArticleDOI
Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Daniel G. Gibson,John I. Glass,Carole Lartigue,Vladimir N. Noskov,Ray-Yuan Chuang,Mikkel A. Algire,Gwynedd A. Benders,Michael G. Montague,Li Ma,Monzia Moodie,Chuck Merryman,Sanjay Vashee,Radha Krishnakumar,Nacyra Assad-Garcia,Cynthia Andrews-Pfannkoch,Evgeniya A. Denisova,Lei Young,Zhi-Qing Qi,Thomas H. Segall-Shapiro,Christopher H. Calvey,Prashanth P. Parmar,Clyde A. Hutchison,Hamilton O. Smith,J. Craig Venter +23 more
TL;DR: The design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new cells that are controlled only by the synthetic chromosome are reported.
Journal ArticleDOI
Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome.
Daniel G. Gibson,Gwynedd A. Benders,Cynthia Andrews-Pfannkoch,Evgeniya A. Denisova,Holly Baden-Tillson,Jayshree Zaveri,Timothy B. Stockwell,Anushka Brownley,David W. Thomas,Mikkel A. Algire,Chuck Merryman,Lei Young,Vladimir N. Noskov,John I. Glass,J. Craig Venter,Clyde A. Hutchison,Hamilton O. Smith +16 more
TL;DR: The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments.
Journal ArticleDOI
One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
Daniel G. Gibson,Gwynedd A. Benders,Kevin C. Axelrod,Jayshree Zaveri,Mikkel A. Algire,Monzia Moodie,Michael G. Montague,J. Craig Venter,Hamilton O. Smith,Clyde A. Hutchison +9 more
TL;DR: Assembly of the synthetic genome from 25 overlapping fragments in a single step using yeast recombination greatly simplifies the assembly of large DNA molecules from both synthetic and natural fragments.
Journal ArticleDOI
Creating bacterial strains from genomes that have been cloned and engineered in yeast.
Carole Lartigue,Sanjay Vashee,Mikkel A. Algire,Ray-Yuan Chuang,Gwynedd A. Benders,Li Ma,Vladimir N. Noskov,Evgeniya A. Denisova,Daniel G. Gibson,Nacyra Assad-Garcia,Nina Alperovich,David W. Thomas,Chuck Merryman,Clyde A. Hutchison,Hamilton O. Smith,J. Craig Venter,John I. Glass +16 more
TL;DR: Methods to accomplish the construction of strains that could not be produced with genetic tools available for this bacterium, including the chemical synthesis, assembly, and cloning of a bacterial genome in yeast, are described.
Journal ArticleDOI
Cloning whole bacterial genomes in yeast
Gwynedd A. Benders,Vladimir N. Noskov,Evgeniya A. Denisova,Carole Lartigue,Daniel G. Gibson,Nacyra Assad-Garcia,Ray-Yuan Chuang,William Carrera,Monzia Moodie,Mikkel A. Algire,Quang Phan,Nina Alperovich,Sanjay Vashee,Chuck Merryman,J. Craig Venter,Hamilton O. Smith,John I. Glass,Clyde A. Hutchison +17 more
TL;DR: The cloning of whole bacterial genomes in the yeast Saccharomyces cerevisiae as single-DNA molecules is reported, and these genomes appear to be stably maintained in a host that has efficient, well-established methods for DNA manipulation.