T
Thomas H. Segall-Shapiro
Researcher at Massachusetts Institute of Technology
Publications - 22
Citations - 3879
Thomas H. Segall-Shapiro is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: Gene & Genome. The author has an hindex of 15, co-authored 20 publications receiving 3375 citations. Previous affiliations of Thomas H. Segall-Shapiro include University of California, San Francisco & California Institute of Technology.
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Journal ArticleDOI
Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Daniel G. Gibson,John I. Glass,Carole Lartigue,Vladimir N. Noskov,Ray-Yuan Chuang,Mikkel A. Algire,Gwynedd A. Benders,Michael G. Montague,Li Ma,Monzia Moodie,Chuck Merryman,Sanjay Vashee,Radha Krishnakumar,Nacyra Assad-Garcia,Cynthia Andrews-Pfannkoch,Evgeniya A. Denisova,Lei Young,Zhi-Qing Qi,Thomas H. Segall-Shapiro,Christopher H. Calvey,Prashanth P. Parmar,Clyde A. Hutchison,Hamilton O. Smith,J. Craig Venter +23 more
TL;DR: The design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new cells that are controlled only by the synthetic chromosome are reported.
Journal ArticleDOI
Escherichia coli "Marionette" strains with 12 highly optimized small-molecule sensors.
TL;DR: A directed evolution approach was applied to optimize a set of 12 small-molecule-responsive biosensors, which led to the engineering of “Marionette” strains of Escherichia coli incorporating these sensors for biotechnological applications.
Journal ArticleDOI
Modular control of multiple pathways using engineered orthogonal T7 polymerases
TL;DR: A design strategy to genetically separate the sensing/circuitry functions from the pathway to be controlled by having the output of the circuit drive the expression of a polymerase, which then activates the pathway from polymerase-specific promoters.
Journal ArticleDOI
A ‘resource allocator’ for transcription based on a highly fragmented T7 RNA polymerase
Thomas H. Segall-Shapiro,Adam J. Meyer,Andrew D. Ellington,Eduardo D. Sontag,Christopher A. Voigt +4 more
TL;DR: It is shown that T7 RNAP can be divided into four fragments that have to be co‐expressed to function, and a resource allocator is built that sets the core fragment concentration, which is then shared by multiple σ fragments.
Design of orthogonal genetic switches based on a crosstalk map of σs, anti-σs, and promoters
Virgil A. Rhodius,Brian D. Sharon,Ekaterina Orlova,Hannah Tabakh,David H. Burkhardt,Kevin Clancy,Todd Peterson,Carol A. Gross,Thomas H. Segall-Shapiro,Amar Ghodasara,Christopher A. Voigt +10 more
TL;DR: The promoter specificities of extracytoplasmic function (ECF) σs as well as the specificity of their interaction with anti‐σs are mapped to create a genome‐scale resource of the properties of ECF ρ and a resource for synthetic biology, where this set of well‐characterized regulatory parts will enable the construction of sophisticated gene expression programs.