L
Li Ma
Researcher at J. Craig Venter Institute
Publications - 13
Citations - 3834
Li Ma is an academic researcher from J. Craig Venter Institute. The author has contributed to research in topics: Genome & Gene. The author has an hindex of 10, co-authored 12 publications receiving 3364 citations.
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Journal ArticleDOI
Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Daniel G. Gibson,John I. Glass,Carole Lartigue,Vladimir N. Noskov,Ray-Yuan Chuang,Mikkel A. Algire,Gwynedd A. Benders,Michael G. Montague,Li Ma,Monzia Moodie,Chuck Merryman,Sanjay Vashee,Radha Krishnakumar,Nacyra Assad-Garcia,Cynthia Andrews-Pfannkoch,Evgeniya A. Denisova,Lei Young,Zhi-Qing Qi,Thomas H. Segall-Shapiro,Christopher H. Calvey,Prashanth P. Parmar,Clyde A. Hutchison,Hamilton O. Smith,J. Craig Venter +23 more
TL;DR: The design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new cells that are controlled only by the synthetic chromosome are reported.
Journal ArticleDOI
Design and synthesis of a minimal bacterial genome
Clyde A. Hutchison,Ray-Yuan Chuang,Vladimir N. Noskov,Nacyra Assad-Garcia,Thomas J. Deerinck,Mark H. Ellisman,John Gill,Krishna Kannan,Bogumil J. Karas,Li Ma,James F. Pelletier,Zhi Qing Qi,R. Alexander Richter,Elizabeth A. Strychalski,Lijie Sun,Yo Suzuki,Billyana Tsvetanova,Kim S. Wise,Hamilton O. Smith,John I. Glass,Chuck Merryman,Daniel G. Gibson,J. Craig Venter +22 more
TL;DR: This work set out to define a minimal cellular genome experimentally by designing and building one, then testing it for viability, and applied whole-genome design and synthesis to the problem of minimizing a cellular genome.
Journal ArticleDOI
Creating bacterial strains from genomes that have been cloned and engineered in yeast.
Carole Lartigue,Sanjay Vashee,Mikkel A. Algire,Ray-Yuan Chuang,Gwynedd A. Benders,Li Ma,Vladimir N. Noskov,Evgeniya A. Denisova,Daniel G. Gibson,Nacyra Assad-Garcia,Nina Alperovich,David W. Thomas,Chuck Merryman,Clyde A. Hutchison,Hamilton O. Smith,J. Craig Venter,John I. Glass +16 more
TL;DR: Methods to accomplish the construction of strains that could not be produced with genetic tools available for this bacterium, including the chemical synthesis, assembly, and cloning of a bacterial genome in yeast, are described.
Journal ArticleDOI
Direct transfer of whole genomes from bacteria to yeast
Bogumil J. Karas,Jelena Jablanovic,Lijie Sun,Li Ma,Gregory M. Goldgof,Gregory M. Goldgof,Jason Stam,Adi Ramon,Micah J. Manary,Elizabeth A. Winzeler,J. Craig Venter,Philip D. Weyman,Daniel G. Gibson,John I. Glass,Clyde A. Hutchison,Hamilton O. Smith,Yo Suzuki +16 more
TL;DR: Direct cell-to-cell transfer of bacterial genomes as large as 1.8 megabases into yeast under conditions that promote cell fusion is demonstrated and it is discovered that removal of restriction endonucleases from donor bacteria resulted in the enhancement of genome transfer.
Journal ArticleDOI
Sequence analysis of a complete 1.66 Mb Prochlorococcus marinus MED4 genome cloned in yeast.
Christian Tagwerker,Christopher L. Dupont,Bogumil J. Karas,Li Ma,Ray-Yuan Chuang,Gwynedd A. Benders,Adi Ramon,Mark Novotny,Michael G. Montague,Pratap Venepally,Daniel Brami,Ariel S. Schwartz,Cynthia Andrews-Pfannkoch,Daniel G. Gibson,John I. Glass,Hamilton O. Smith,J. Craig Venter,Clyde A. Hutchison +17 more
TL;DR: Evidence of transformation, replication and maintenance of this 1.66 Mb intact bacterial genome in S. cerevisiae is provided.