C
Clyde A. Hutchison
Researcher at J. Craig Venter Institute
Publications - 137
Citations - 26619
Clyde A. Hutchison is an academic researcher from J. Craig Venter Institute. The author has contributed to research in topics: Gene & Genome. The author has an hindex of 52, co-authored 136 publications receiving 23912 citations. Previous affiliations of Clyde A. Hutchison include Torrey Pines Institute for Molecular Studies & University of North Carolina at Chapel Hill.
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Journal ArticleDOI
Enzymatic assembly of DNA molecules up to several hundred kilobases
Daniel G. Gibson,Lei Young,Ray-Yuan Chuang,J. Craig Venter,Clyde A. Hutchison,Hamilton O. Smith +5 more
TL;DR: An isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5′ exonuclease, a DNA polymerase and a DNA ligase is described.
Journal ArticleDOI
The minimal gene complement of Mycoplasma genitalium
Claire M. Fraser,Jeannine D. Gocayne,Owen White,Mark Raymond Adams,Rebecca A. Clayton,Robert D. Fleischmann,Carol J. Bult,Anthony R. Kerlavage,Granger G. Sutton,Jenny M. Kelley,Janice L. Fritchman,Janice Weidman,Keith V. Small,Mina Sandusky,Joyce Fuhrmann,David Nguyen,Teresa Utterback,D. Saudek,Cheryl Phillips,Joseph M. Merrick,J F Tomb,Brian Dougherty,Kenneth F. Bott,Ping Chuan Hu,Thomas Lucier,Scott N. Peterson,Hamilton O. Smith,Clyde A. Hutchison,J. Craig Venter +28 more
TL;DR: Comparison of the Mycoplasma genitalium genome to that of Haemophilus influenzae suggests that differences in genome content are reflected as profound differences in physiology and metabolic capacity between these two organisms.
Journal ArticleDOI
Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Daniel G. Gibson,John I. Glass,Carole Lartigue,Vladimir N. Noskov,Ray-Yuan Chuang,Mikkel A. Algire,Gwynedd A. Benders,Michael G. Montague,Li Ma,Monzia Moodie,Chuck Merryman,Sanjay Vashee,Radha Krishnakumar,Nacyra Assad-Garcia,Cynthia Andrews-Pfannkoch,Evgeniya A. Denisova,Lei Young,Zhi-Qing Qi,Thomas H. Segall-Shapiro,Christopher H. Calvey,Prashanth P. Parmar,Clyde A. Hutchison,Hamilton O. Smith,J. Craig Venter +23 more
TL;DR: The design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new cells that are controlled only by the synthetic chromosome are reported.
Journal ArticleDOI
Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome.
Daniel G. Gibson,Gwynedd A. Benders,Cynthia Andrews-Pfannkoch,Evgeniya A. Denisova,Holly Baden-Tillson,Jayshree Zaveri,Timothy B. Stockwell,Anushka Brownley,David W. Thomas,Mikkel A. Algire,Chuck Merryman,Lei Young,Vladimir N. Noskov,John I. Glass,J. Craig Venter,Clyde A. Hutchison,Hamilton O. Smith +16 more
TL;DR: The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments.
Journal ArticleDOI
Design and synthesis of a minimal bacterial genome
Clyde A. Hutchison,Ray-Yuan Chuang,Vladimir N. Noskov,Nacyra Assad-Garcia,Thomas J. Deerinck,Mark H. Ellisman,John Gill,Krishna Kannan,Bogumil J. Karas,Li Ma,James F. Pelletier,Zhi Qing Qi,R. Alexander Richter,Elizabeth A. Strychalski,Lijie Sun,Yo Suzuki,Billyana Tsvetanova,Kim S. Wise,Hamilton O. Smith,John I. Glass,Chuck Merryman,Daniel G. Gibson,J. Craig Venter +22 more
TL;DR: This work set out to define a minimal cellular genome experimentally by designing and building one, then testing it for viability, and applied whole-genome design and synthesis to the problem of minimizing a cellular genome.