M
Mikkel A. Algire
Researcher at J. Craig Venter Institute
Publications - 15
Citations - 4514
Mikkel A. Algire is an academic researcher from J. Craig Venter Institute. The author has contributed to research in topics: Genome & Gene. The author has an hindex of 9, co-authored 14 publications receiving 4108 citations.
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Journal ArticleDOI
Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Daniel G. Gibson,John I. Glass,Carole Lartigue,Vladimir N. Noskov,Ray-Yuan Chuang,Mikkel A. Algire,Gwynedd A. Benders,Michael G. Montague,Li Ma,Monzia Moodie,Chuck Merryman,Sanjay Vashee,Radha Krishnakumar,Nacyra Assad-Garcia,Cynthia Andrews-Pfannkoch,Evgeniya A. Denisova,Lei Young,Zhi-Qing Qi,Thomas H. Segall-Shapiro,Christopher H. Calvey,Prashanth P. Parmar,Clyde A. Hutchison,Hamilton O. Smith,J. Craig Venter +23 more
TL;DR: The design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new cells that are controlled only by the synthetic chromosome are reported.
Journal ArticleDOI
Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome.
Daniel G. Gibson,Gwynedd A. Benders,Cynthia Andrews-Pfannkoch,Evgeniya A. Denisova,Holly Baden-Tillson,Jayshree Zaveri,Timothy B. Stockwell,Anushka Brownley,David W. Thomas,Mikkel A. Algire,Chuck Merryman,Lei Young,Vladimir N. Noskov,John I. Glass,J. Craig Venter,Clyde A. Hutchison,Hamilton O. Smith +16 more
TL;DR: The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments.
Journal ArticleDOI
One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
Daniel G. Gibson,Gwynedd A. Benders,Kevin C. Axelrod,Jayshree Zaveri,Mikkel A. Algire,Monzia Moodie,Michael G. Montague,J. Craig Venter,Hamilton O. Smith,Clyde A. Hutchison +9 more
TL;DR: Assembly of the synthetic genome from 25 overlapping fragments in a single step using yeast recombination greatly simplifies the assembly of large DNA molecules from both synthetic and natural fragments.
Journal ArticleDOI
Creating bacterial strains from genomes that have been cloned and engineered in yeast.
Carole Lartigue,Sanjay Vashee,Mikkel A. Algire,Ray-Yuan Chuang,Gwynedd A. Benders,Li Ma,Vladimir N. Noskov,Evgeniya A. Denisova,Daniel G. Gibson,Nacyra Assad-Garcia,Nina Alperovich,David W. Thomas,Chuck Merryman,Clyde A. Hutchison,Hamilton O. Smith,J. Craig Venter,John I. Glass +16 more
TL;DR: Methods to accomplish the construction of strains that could not be produced with genetic tools available for this bacterium, including the chemical synthesis, assembly, and cloning of a bacterial genome in yeast, are described.
Journal ArticleDOI
Synthetic Generation of Influenza Vaccine Viruses for Rapid Response to Pandemics
Philip R. Dormitzer,Pirada Suphaphiphat,Daniel G. Gibson,David E. Wentworth,Timothy B. Stockwell,Mikkel A. Algire,Nina Alperovich,Mario Barro,David M. Brown,Stewart Craig,Brian M. Dattilo,Evgeniya A. Denisova,Ivna De Souza,Markus Eickmann,Vivien G. Dugan,Annette Ferrari,Raul Gomila,Liqun Han,Casey Judge,Sarthak Mane,Mikhail Matrosovich,Chuck Merryman,Giuseppe Palladino,Gene A. Palmer,Terika Spencer,Thomas Strecker,Heidi Trusheim,Jennifer Uhlendorff,Yingxia Wen,Anthony Yee,Jayshree Zaveri,Bin Zhou,Stephan Becker,Armen Donabedian,Peter W. Mason,John I. Glass,Rino Rappuoli,J. Craig Venter +37 more
TL;DR: A synthetic approach that very rapidly generated vaccine viruses from sequence data was developed, which could shave weeks off the time needed for vaccine manufacture and put us in a much better position to respond quickly to a sudden pandemic.