J
John I. Glass
Researcher at J. Craig Venter Institute
Publications - 112
Citations - 13168
John I. Glass is an academic researcher from J. Craig Venter Institute. The author has contributed to research in topics: Genome & Gene. The author has an hindex of 40, co-authored 101 publications receiving 11835 citations. Previous affiliations of John I. Glass include Eli Lilly and Company & University of North Carolina at Chapel Hill.
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Journal ArticleDOI
Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Daniel G. Gibson,John I. Glass,Carole Lartigue,Vladimir N. Noskov,Ray-Yuan Chuang,Mikkel A. Algire,Gwynedd A. Benders,Michael G. Montague,Li Ma,Monzia Moodie,Chuck Merryman,Sanjay Vashee,Radha Krishnakumar,Nacyra Assad-Garcia,Cynthia Andrews-Pfannkoch,Evgeniya A. Denisova,Lei Young,Zhi-Qing Qi,Thomas H. Segall-Shapiro,Christopher H. Calvey,Prashanth P. Parmar,Clyde A. Hutchison,Hamilton O. Smith,J. Craig Venter +23 more
TL;DR: The design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new cells that are controlled only by the synthetic chromosome are reported.
Journal ArticleDOI
A Whole-Cell Computational Model Predicts Phenotype from Genotype
Jonathan R. Karr,Jayodita C. Sanghvi,Derek N. Macklin,Miriam V. Gutschow,Jared M. Jacobs,Benjamin Bolival,Nacyra Assad-Garcia,John I. Glass,Markus W. Covert +8 more
TL;DR: A whole-cell computational model of the life cycle of the human pathogen Mycoplasma genitalium that includes all of its molecular components and their interactions is reported, concluding that comprehensive whole- cell models can be used to facilitate biological discovery.
Journal ArticleDOI
Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome.
Daniel G. Gibson,Gwynedd A. Benders,Cynthia Andrews-Pfannkoch,Evgeniya A. Denisova,Holly Baden-Tillson,Jayshree Zaveri,Timothy B. Stockwell,Anushka Brownley,David W. Thomas,Mikkel A. Algire,Chuck Merryman,Lei Young,Vladimir N. Noskov,John I. Glass,J. Craig Venter,Clyde A. Hutchison,Hamilton O. Smith +16 more
TL;DR: The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments.
Journal ArticleDOI
Design and synthesis of a minimal bacterial genome
Clyde A. Hutchison,Ray-Yuan Chuang,Vladimir N. Noskov,Nacyra Assad-Garcia,Thomas J. Deerinck,Mark H. Ellisman,John Gill,Krishna Kannan,Bogumil J. Karas,Li Ma,James F. Pelletier,Zhi Qing Qi,R. Alexander Richter,Elizabeth A. Strychalski,Lijie Sun,Yo Suzuki,Billyana Tsvetanova,Kim S. Wise,Hamilton O. Smith,John I. Glass,Chuck Merryman,Daniel G. Gibson,J. Craig Venter +22 more
TL;DR: This work set out to define a minimal cellular genome experimentally by designing and building one, then testing it for viability, and applied whole-genome design and synthesis to the problem of minimizing a cellular genome.
Journal ArticleDOI
Essential genes of a minimal bacterium
John I. Glass,Nacyra Assad-Garcia,Nina Alperovich,Shibu Yooseph,Matthew R. Lewis,Mahir Maruf,Clyde A. Hutchison,Hamilton O. Smith,J. Craig Venter +8 more
TL;DR: Using global transposon mutagenesis, this work identifies 382 of the 482 M. genitalium protein-coding genes as essential, plus five sets of disrupted genes that encode proteins with potentially redundant essential functions, such as phosphate transport.