Hans J. Tanke

TL;DR: A protocol that enables the visualization and tracking of telomeres in living cells by hybridization with a fluorescent peptide nucleic acid (PNA) probe is described and a method that allows the detection of abundant endogenous RNAs in live cells by microinjecting fluorescently labeled complementary 2'-O-methyl RNA probes is described.

TL;DR: Investigation of the feasibility of automated counting of in situ hybridization signals (ISH) in interphase cells isolated from paraffin embedded prostate tissue found it to be useful for the evaluation of chromosomal aberrations in prostate tumor cells, provided that the counts are visually confirmed.

TL;DR: A method that uses a model of the bleaching process to correct motion and that the model based fluorescence intensity and attenuation estimates can be interpreted easily is described, making it suitable for unsupervised batch processing of large data series.

TL;DR: Electropermeabilized neutrophils showed an equal sensitivity to Ca2+ and a graded sensitivity to GTP gamma S, and the light scatter pattern of the population changed indicating that the cells were gradually sensitive to G TP gamma S.

TL;DR: High sensitivity was achieved by optimizing the conditions of the hybridization procedure, the immunochemical detection and the peroxidase/luminol reaction, which resulted in the reproducible detection of 10-30 femtogram of target DNA on blots within minutes when a cooled charge coupled device (CCD) camera was used to record the luminescence signal.