Helenia Ansuini

TL;DR: It is discovered that the hepatitis C virus (HCV) envelope glycoprotein E2 binds to human hepatoma cell lines independently of the previously proposed HCV receptor CD81, and the receptor responsible for E2 binding to human hepatic cells is identified as the human scavenger receptor class B type I (SR‐BI).

TL;DR: E2 proteins from different isolates displayed a profile of binding to human hepatic cells different from that observed on Molt-4 cells or isolated recombinant CD81, indicating that additional factors are involved in viral recognition by target liver cells.

TL;DR: A specific and direct role for the identified residues in binding HCV and mediating virus entry is suggested and the hypothesis that new therapeutic strategies aimed at interfering with virus/SR-BI recognition are feasible is supported.

TL;DR: The strategy described represents a powerful tool to identify proteins that bind to DNA regulatory elements and an array of multiple adjacent DNA-binding sites sharing a common consensus motif was identified.

TL;DR: A novel lambda vector for display of exogenous peptides at the C-terminus of the capsid D protein is described, which allows efficient in vivo biotinylation of the chimeric phage during amplification.