Hiroshi Kakeshita

TL;DR: To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, a systematic inactivation of Bacillus subtilis genes was carried out and most genes involved in the Embden–Meyerhof–Parnas pathway are essential.

TL;DR: Although their functions are unknown, gene expression profiles at several time points showed that most of the genes except for bsrD were expressed during the vegetative phase, but undetectable during the stationary phase, and mapping the 5' ends of the 6 small RNAs revealed that the genes for BsrE, BsrF, B srG, BSRH, and BsrI RNAs are preceded by a recognition site for RNA polymerase sigma factor sigma(A).

TL;DR: In vitro assay system indicated that co-operation between the SRP–protein targeting system to the cell membrane and the Sec protein translocation machinery across the cytoplasmic membrane constitutes the major protein secretion pathway in B. subtilis.

TL;DR: To determine the signal recognition particle (SRP)-SRP receptor (Srb) system in Bacillus subtilis (Bs), the Bs srb gene was cloned, which encodes a homologue of the mammalian SRP receptor alpha-subunit and the gene organization was analyzed.

TL;DR: A phylogenetic comparison of the sequence of the BS190RNA gene with sequences from the databases suggests that RNA related to BS190 RNA appears to be encoded in the genomes of Bacillus halodurans and Listeria monocytogenes.