Huan Wang

TL;DR: These findings support a two-tiered approach combining anticancer agents that eliminate rapidly proliferating melanoma cells with inhibitors of the drug-resistant slow-cycling subpopulation, and support an intrinsic multidrug resistance based on the survival of a tumor cell subpopulation.

TL;DR: It is reported that Heat Shock Protein 90 (Hsp90)-directed protein folding in mitochondria controls central metabolic networks in tumor cells, including the electron transport chain, citric acid cycle, fatty acid oxidation, amino acid synthesis, and cellular redox status.

TL;DR: High reproducibility of complex proteomes, such as human cancer cell lysates, readily can be achieved when using multidimensional separation methods with good depth of analysis when factors such as database redundancy and use of rigid scoring thresholds were considered.

TL;DR: High pH reverse-phase HPLC exhibited the highest peptide resolution and yielded the best depth of analysis with detection of the largest number of known low-abundance proteins for a given level of fractionation.

TL;DR: Analysis of larger pools of patient sera suggested that all 11 novel biomarker candidates selected by the multi-step prioritization and verification process are promising candidate biomarkers that should be further evaluated on individual patient blood samples.