Kaye D. Speicher

TL;DR: Central analysis determined missed identifications, environmental contamination, database matching and curation of protein identifications as sources of problems in liquid chromatography–mass spectrometry–based proteomics.

TL;DR: An optimized in-geltrypsin digestion strategy in which proteins in 1.0-mm-thick gels are stained with Coomassie blue or Ruby Red, digested overnight with modified trypsin, and extracted one or two times with small volumes of aqueous buffer is suggested.

TL;DR: A subset of membrane proteins are defined as sheddome candidates, forming the basis for further studies examining the impact of ectodomain shedding on platelet function.

TL;DR: Ten internalized DRM proteins show varied lipid and peptidic anchors indicating that, contrary to the prevailing model of apicomplexan vacuole formation, DRM association, rather than lipid anchors, provides the preferred criteria for protein recruitment to the malarial vacuoles.

TL;DR: It is reported that Heat Shock Protein 90 (Hsp90)-directed protein folding in mitochondria controls central metabolic networks in tumor cells, including the electron transport chain, citric acid cycle, fatty acid oxidation, amino acid synthesis, and cellular redox status.