J
J. Wade Harper
Researcher at Harvard University
Publications - 292
Citations - 75309
J. Wade Harper is an academic researcher from Harvard University. The author has contributed to research in topics: Ubiquitin ligase & Ubiquitin. The author has an hindex of 109, co-authored 279 publications receiving 66267 citations. Previous affiliations of J. Wade Harper include University of Wisconsin-Madison & Institute of Cancer Research.
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Journal ArticleDOI
SGT1 Encodes an Essential Component of the Yeast Kinetochore Assembly Pathway and a Novel Subunit of the SCF Ubiquitin Ligase Complex
TL;DR: Genetic and phenotypic analyses of sgt1-3 (G2 allele) mutants support an essential role in kinetochore function, suggesting that the function of SGT1 is conserved in evolution.
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Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging
TL;DR: This work identifies a divergent E1 in vertebrates and sea urchin, Uba6, which specifically activates ubiquitin but not other UBLs in vitro and in vivo, and reveals unexpected complexity in the pathways that control the conjugation of Ubiquitin.
Journal ArticleDOI
Anaphase initiation is regulated by antagonistic ubiquitination and deubiquitination activities
Frank Stegmeier,Michael Rape,Michael Rape,Viji M. Draviam,Viji M. Draviam,Grzegorz Nalepa,Mathew E. Sowa,Xiaolu L. Ang,E. Robert McDonald,Mamie Z. Li,Gregory J. Hannon,Peter K. Sorger,Peter K. Sorger,Marc W. Kirschner,J. Wade Harper,Stephen J. Elledge +15 more
TL;DR: A dynamic balance of ubiquitination and deubiquitination by the APC and USP44 contributes to the generation of the switch-like transition controlling anaphase entry, analogous to the way that phosphorylation and dephosphorylation of Cdk1 by Wee1 and Cdc25 controls entry into mitosis.
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Cell cycle-regulated phosphorylation of p220(NPAT) by cyclin E/Cdk2 in Cajal bodies promotes histone gene transcription.
Tianlin Ma,Brian A. Van Tine,Yue Wei,Michelle D. Garrett,David M. Nelson,Peter D. Adams,Jin Wang,Jun Qin,Louise T. Chow,J. Wade Harper +9 more
TL;DR: Results strongly suggest that p220(NPAT) links cyclical cyclin E/Cdk2 kinase activity to replication-dependent histone gene transcription.
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SCFβ-TRCP links Chk1 signaling to degradation of the Cdc25A protein phosphatase
Jianping Jin,Takahiro Shirogane,Lai Xu,Grzegorz Nalepa,Grzegorz Nalepa,Jun Qin,Stephen J. Elledge,J. Wade Harper +7 more
TL;DR: A requirement for SCFbeta-TRCP in Cdc25A turnover during an unperturbed cell cycle and in response to DNA damage is demonstrated and roles for an additional kinase(s) in Chk1-dependent Cdc 25A turnover are suggested.