Jaakko Itkonen

TL;DR: This article aims to provide a systematic and quantitative view on the pharmacokinetic factors in drug delivery to the posterior eye segment, based on the literature and unpublished data from the authors' laboratory.

TL;DR: Time-gated Raman spectroscopy showed to be a powerful tool to evaluate protein production and correct folding within living E. coli cells during the cultivation.

TL;DR: The results highlight the combination of codon optimization and screening soluble fusion tags as a successful strategy for high yielding soluble expression of hCNTF in E. coli.

TL;DR: In this article, the authors characterized recombinant human Ciliary neurotrophic factor (rhCNTF) in vitro and evaluated the thermodynamic and oligomeric status of the protein during storage.

TL;DR: Split-intein mediated capture, protein trans splicing followed by light triggered release, and bioconjugation for proteins synthesized in cell free systems might be performed in an integrated workflow resulting in the fast production of the target protein.