J
James D. Young
Researcher at University of Alberta
Publications - 241
Citations - 16156
James D. Young is an academic researcher from University of Alberta. The author has contributed to research in topics: Nucleoside & Nucleoside transporter. The author has an hindex of 63, co-authored 240 publications receiving 15574 citations. Previous affiliations of James D. Young include Cornell University & Cross Cancer Institute.
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Journal ArticleDOI
Identification of a novel membrane transporter associated with intracellular membranes by phenotypic complementation in the yeast Saccharomyces cerevisiae.
TL;DR: A partial mouse cDNA was isolated by its ability to functionally complement a thymidine transport deficiency in plasma membranes of the yeast, Saccharomyces cerevisiae as discussed by the authors.
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Acquisition of human concentrative nucleoside transporter 2 (hcnt2) activity by gene transfer confers sensitivity to fluoropyrimidine nucleosides in drug-resistant leukemia cells.
TL;DR: HCNT2 is an important determinant of cytotoxicity of this class of compounds in vivo, and chemosensitivity was restored partially for cladribine, completely for 5-fluorouridine and5-fluoro-2'-deoxyuridine, whereas there was little effect on chemos sensitivity for fludarabine, 7-deazaadenosine, or cytarabine.
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Biodistribution and uptake of 3'-deoxy-3'-fluorothymidine in ENT1-knockout mice and in an ENT1-knockdown tumor model.
Robert J. Paproski,Melinda Wuest,Hans-Sonke Jans,Kathryn Graham,Wendy P. Gati,S. A. McQuarrie,Alexander J.B. McEwan,John R. Mercer,James D. Young,Carol E. Cass +9 more
TL;DR: Loss of ENT1 activity significantly affected 18F-FLT biodistribution in mice and 18F -FLT uptake in xenograft tumors, suggesting that nucleoside transporters are important mediators of 18F+/− uptake in normal and transformed cells.
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Reconstitution studies of the human erythrocyte nucleoside transporter.
TL;DR: In the present study, the NBMPR-binding protein was extracted from protein-depleted human erythrocyte "ghosts" with Triton X-100 and reconstituted into soybean phospholipid vesicles by a freeze-thaw-sonication procedure, which implicate band 4.5 polypeptides in both nucleoside and sugar permeation.
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Differential Transport of Cytosine-Containing Nucleosides by Recombinant Human Concentrative Nucleoside Transporter Protein hCNT1
Kathryn Graham,Jackie Leithoff,Imogen R. Coe,Delores Mowles,John R. Mackey,James D. Young,Carol E. Cass +6 more
TL;DR: Uridine transport was inhibited 89, 32 and 11%, respectively, by 500 μM gem citabine, cytarabine and lamivudine, demonstrating that, unlike gemcitabine (a high-affinity hCNT1 permeant), cytarABine and LamivUDine are poor hC NT1 permeants.