J
James K. Nuñez
Researcher at University of California, Berkeley
Publications - 17
Citations - 4202
James K. Nuñez is an academic researcher from University of California, Berkeley. The author has contributed to research in topics: CRISPR & Cas9. The author has an hindex of 12, co-authored 16 publications receiving 3110 citations. Previous affiliations of James K. Nuñez include University of California, San Francisco & Howard Hughes Medical Institute.
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Journal ArticleDOI
Biology and Applications of CRISPR Systems: Harnessing Nature’s Toolbox for Genome Engineering
TL;DR: Recent advances in understanding the diverse mechanisms by which Cas proteins respond to foreign nucleic acids are discussed and how these systems have been harnessed for precision genome manipulation in a wide array of organisms.
Journal ArticleDOI
A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response.
Britt Adamson,Thomas M. Norman,Marco Jost,Min Y. Cho,James K. Nuñez,Yuwen Chen,Jacqueline E. Villalta,Luke A. Gilbert,Max A. Horlbeck,Marco Y. Hein,Ryan A. Pak,Andrew N. Gray,Carol A. Gross,Atray Dixit,Oren Parnas,Aviv Regev,Jonathan S. Weissman +16 more
TL;DR: Perturb-seq as mentioned in this paper combines droplet-based single-cell RNA-seq with a strategy for barcoding CRISPR-mediated perturbations, allowing many perturbation to be profiled in pooled format.
A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response
Britt Adamson,Thomas M. Norman,Marco Jost,Min Y. Cho,James K. Nuñez,Yuwen Chen,Jacqueline E. Villalta,Luke A. Gilbert,Max A. Horlbeck,Marco Y. Hein,Ryan A. Pak,Andrew N. Gray,Carol A. Gross,Oren Parnas,Jonathan S. Weissman,Atray Dixit,Aviv Regev +16 more
TL;DR: Insight is provided into how the three sensors of ER homeostasis monitor distinct types of stress and the ability of Perturb-seq to dissect complex cellular responses are highlighted.
Journal ArticleDOI
Cas1–Cas2 complex formation mediates spacer acquisition during CRISPR–Cas adaptive immunity
James K. Nuñez,Philip J. Kranzusch,Jonas Noeske,Addison V. Wright,Christopher W. Davies,Jennifer A. Doudna +5 more
TL;DR: It is shown that Cas1 and Cas2 from Escherichia coli form a stable complex that is essential for spacer acquisition and determined the 2.3-Å-resolution crystal structure of the Cas1–Cas2 complex, suggesting a mechanism by which Cas1-2 complexes specify sites of CRISPR spacer integration.
Journal ArticleDOI
Pervasive functional translation of noncanonical human open reading frames
Jin Chen,Andreas-David Brunner,J. Zachery Cogan,James K. Nuñez,Alexander P. Fields,Britt Adamson,Daniel N. Itzhak,Jason Y. Li,Matthias Mann,Matthias Mann,Manuel D. Leonetti,Jonathan S. Weissman +11 more
TL;DR: A systematic CRISPR-based screening strategy is exploited to identify hundreds of noncanonical CDSs that are essential for cellular growth and whose disruption elicits specific, robust transcriptomic and phenotypic changes in human cells.