James Lu

TL;DR: F Fourier analysis of the two-level p-multigrid algorithm for convection-diffusion shows that element line Jacobi presents a significant improvement over element Jacobi especially for high Reynolds number flows and stretched grids.

TL;DR: Systemic delivery in mice of the complexes of a short interfering RNA against a model gene, firefly luciferase, and PEI25 or PEI87 afforded a 77% and 93% suppression of the gene expression in the lungs, respectively, validation of the potential of such linear, fully deacylated PEIs in gene therapy for lung diseases.

TL;DR: Three ontologies created specifically to address the needs of the systems biology community are described, including the Systems Biology Ontology, which provides semantic information about the model components, and the Kinetic Simulation Algorithm Ontology and the Terminology for the Description of Dynamics, which categorizes dynamical features of the simulation results and general systems behavior.

TL;DR: Cross-linking of small PEIs with judiciously designed amide- and ester-bearing linkers boosts their gene delivery efficiency both in vitro and in vivo without increasing the cytotoxicity.

TL;DR: In this article, the authors investigated the mechanisms by which a cationic lipid carrier mediates siRNA delivery into mammalian cells and showed that approximately 95% of siRNA lipoplexes enter the cells through endocytosis and persist in endolysosomes for a prolonged period of time.