Jérôme Ruel

TL;DR: The functional consequences of inhibiting glutamate uptake are investigated by recording cochlear potentials during intracochlear perfusion with either l‐trans‐pyrrolidine‐2,4‐dicarboxylic acid or dihydrokainate, and cochleas displayed a swelling of the afferent endings typical of excitotoxicity.

TL;DR: The first demonstration that transient knockout of GluR2 subunit in adult animal modifies excitatory synaptic transmission in vivo is demonstrated, supporting the use of the antisense strategy as a powerful tool for blocking expression of any gene in the cochlea.

TL;DR: A two-photon imaging technique in cochlear and vestibular slice preparations from neonatal rats and turtles is developed based on a retrograde labeling of afferent nerve endings with high-affinity calcium-sensitive dyes, which results in typical recordings and potential interests for pharmacological studies of inner ear sensory cell synapses are discussed.

TL;DR: An in vitro model of mechanotransduction that provides an opportunity to explore the properties of mechanosensitive channels in mammalian sensory neurons is described and the mechano-clamp method is described, which allows applying local force on plasma membrane of whole-cell patch-clamped sensory neurons.

TL;DR: The expression of different isoforms of microtubule-associated proteins 2 (MAP2), including the low molecular weight form MAP2c present mainly in developing neurons, was investigated in the primary auditory neurons after alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid perfusion in the guinea pig cochlea.