Jinhui Zhong

TL;DR: This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans, and recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components.

TL;DR: The substantial number of new interactions discovered here supports the conclusion that previous interaction mapping studies were far from complete and that many more interactions remain to be found.

TL;DR: Interaction mating is described, a two-hybrid variation that can be adapted to most versions of the system and that can simplify and facilitate most two- Hybrid experiments.

TL;DR: An efficient two-hybrid strategy to facilitate construction of protein interaction maps on a genome-wide scale that has several advantages over previously described approaches, including the ability to detect interactions with proteins that inhibit yeast growth or that activate transcription as BD fusions.

TL;DR: A conditional expression system that takes advantage of the yeast MAL62 promoter (MAL62p), which can be controlled by adding maltose or glucose to the growth medium to induce or repress transcription, and a mating scheme is used to dramatically increase the dynamic range of expression levels possible.