Book ChapterDOI
Interaction mating methods in two-hybrid systems
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TLDR
Interaction mating is described, a two-hybrid variation that can be adapted to most versions of the system and that can simplify and facilitate most two- Hybrid experiments.Abstract:
Publisher Summary The yeast two-hybrid system is a powerful assay for protein–protein interactions. The two proteins to be tested for interaction are expressed as hybrids in the nucleus of a yeast cell. One of the proteins is fused to the deoxyribonucleic acid (DNA)-binding domain (DBD) of a transcription factor and the other is fused to a transcription activation domain (AD). If the two hybrid proteins interact, they reconstitute a functional transcription factor that activates one or more reporter genes that contain binding sites for the DBD. This simple assay has been widely used to identify new interacting proteins from libraries, to test interactions between small and large sets of proteins, to map protein networks, and to address the functions of individual proteins and protein interactions. This chapter describes interaction mating, a two-hybrid variation that can be adapted to most versions of the system and that can simplify and facilitate most two-hybrid experiments.read more
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Role of SCF ubiquitin-ligase and the COP9 signalosome in the N gene-mediated resistance response to Tobacco mosaic virus.
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TL;DR: A genome-scale specificity and interaction map for yeast SH3 domain-containing proteins reveal how family members show selective binding to target proteins and predicts the dynamic localization of new candidate endocytosis proteins.
Patent
Multi-chain eukaryotic display vectors and uses thereof
TL;DR: In this article, a eukaryotic expression vector capable of displaying a multi-chain polypeptide on the surface of a host cell is provided, such that the biological activity of the multi-chains polypeptic is exhibited at the surface surface of the host cell.
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Identification of New Protein Interactions between Dengue Fever Virus and Its Hosts, Human and Mosquito
Dumrong Mairiang,Huamei Zhang,Ann Sodja,T. M. Murali,Prapat Suriyaphol,Prida Malasit,Prida Malasit,Thawornchai Limjindaporn,Russell L. Finley +8 more
TL;DR: High-throughput yeast two-hybrid screening is used to identify mosquito and human proteins that physically interact with dengue proteins and identified a subset of the host-dengue interactions that are now supported by multiple forms of evidence, which should be useful for understanding the interplay between d Dengue and its hosts.
References
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