J
Jiuxin Qu
Researcher at University of California, San Francisco
Publications - 4
Citations - 279
Jiuxin Qu is an academic researcher from University of California, San Francisco. The author has contributed to research in topics: Microbiome & CRISPR interference. The author has an hindex of 3, co-authored 4 publications receiving 174 citations. Previous affiliations of Jiuxin Qu include University of California, Berkeley & South University of Science and Technology of China.
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Journal ArticleDOI
Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.
Jason M. Peters,Jason M. Peters,Byoung-Mo Koo,Ramiro Patino,Gary E. Heussler,Cameron C. Hearne,Jiuxin Qu,Yuki F. Inclan,John S. Hawkins,Candy H. S. Lu,Melanie R. Silvis,M. Michael Harden,Hendrik Osadnik,Joseph E. Peters,Joanne N. Engel,Rachel J. Dutton,Alan D. Grossman,Carol A. Gross,Oren S. Rosenberg +18 more
TL;DR: ‘Mobile-CRISpri’ is established, a suite of CRISPRi systems that combines modularity, stable genomic integration and ease of transfer to diverse bacteria by conjugation that enables genetic investigations in non-model bacteria.
Journal ArticleDOI
Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs.
Kale Kundert,James E. Lucas,Kyle E. Watters,Christof Fellmann,Andrew H. Ng,Benjamin M. Heineike,Christina M. Fitzsimmons,Christina M. Fitzsimmons,Benjamin L. Oakes,Jiuxin Qu,Neha K. Prasad,Oren S. Rosenberg,David F. Savage,Hana El-Samad,Jennifer A. Doudna,Tanja Kortemme +15 more
TL;DR: It is shown that CRISPR-Cas9-based gene repression (CRISPRi) can be either activated or deactivated in a dose-dependent fashion over a >10-fold dynamic range in response to two different small-molecule ligands.
Journal ArticleDOI
Modulating Pathogenesis with Mobile-CRISPRi.
Jiuxin Qu,Neha K. Prasad,Michelle Yu,Shuyan Chen,Amy Lyden,Nadia Herrera,Melanie R. Silvis,Emily D. Crawford,Mark R. Looney,Jason M. Peters,Oren S. Rosenberg +10 more
TL;DR: The first application of Mobile-CRISPRi is provided to study conditionally essential virulence genes in mouse models of lung infection through partial gene perturbation and it is demonstrated that knockdown of a CE gene encoding the type III secretion system activator ExsA blocks effector protein secretion in culture and attenuates virulence in mice.
Posted ContentDOI
Modulating pathogenesis with Mobile-CRISPRi
Jiuxin Qu,Jiuxin Qu,Neha K. Prasad,Michelle Yu,Mark R. Looney,Shuyan Chen,Shuyan Chen,Amy Lyden,Emily D. Crawford,Melanie R. Silvis,Jason M. Peters,Jason M. Peters,Oren S. Rosenberg +12 more
TL;DR: The first application of Mobile-CRISPRi is provided to study virulence life-style genes in mouse models of lung infection through partial gene perturbation, and it is demonstrated that knockdown of a VLG encoding the type III secretion system (T3SS) activator ExsA blocks effector protein secretion in culture and attenuates virulence in mice.