Joana Ribeiro

TL;DR: A modified 2D/3D protocol is reported for generating hPSC-derived neural retinal vesicles with well-formed ONL-like structures containing cones and rods bearing inner segments and connecting cilia, nascent outer segments, and presynaptic structures that recapitulates human photoreceptor development, allowing the isolation and transplantation of a pure population of stage-matched cones.

TL;DR: It is found that material transfer accounts for a significant proportion of rescued cells following cone transplantation into non-degenerative hosts, and confirms the occurrence of photoreceptor integration in certain models of retinal degeneration and demonstrates the importance of the host environment in determining transplantation outcome.

TL;DR: Gene replacement in rd1 mice that are devoid of the mutation in Gpr179 successfully restores the function of both photoreceptors and bipolar cells, which is maintained for up to 13 months.

TL;DR: Analysis revealed that the use of bioreactors results in improved laminar stratification as well as an increase in the yield of photoreceptor cells bearing cilia and nascent outer-segment-like structures.

TL;DR: It is concluded that the demand for RPE65 in humans is not fully met by current vectors, and it is predicted that a more powerful vector will provide more durable benefit.