J
Joerg Hoernschemeyer
Researcher at University of Bonn
Publications - 9
Citations - 1071
Joerg Hoernschemeyer is an academic researcher from University of Bonn. The author has contributed to research in topics: Recombinant DNA & Glycoprotein. The author has an hindex of 7, co-authored 9 publications receiving 1003 citations.
Papers
More filters
Journal ArticleDOI
Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation.
Richard Wubbolts,Rachel S. Leckie,Peter T. M. Veenhuizen,Guenter Schwarzmann,Wiebke Möbius,Joerg Hoernschemeyer,J W Slot,Hans J. Geuze,Willem Stoorvogel +8 more
TL;DR: Data suggest that recruitment of membrane proteins from the limiting membranes into the internal vesicles of multivesicular bodies may involve their incorporation into tetraspanin-containing detergent-resistant membrane domains.
Journal ArticleDOI
Human acid sphingomyelinase - Assignment of the disulfide bond pattern
Stephanie Lansmann,Christina G. Schuette,Oliver Bartelsen,Joerg Hoernschemeyer,Thomas Linke,Judith Weisgerber,Konrad Sandhoff +6 more
TL;DR: The hypothesis that haSMase possesses an intramolecular SAP-type activator domain as predicted by sequence comparison is supported and an additional analysis of ha SMase isolated from human placenta shows that the recombinant and the native human protein possess an identical disulfide structure.
Journal ArticleDOI
Photoaffinity labelling of the human GM2-activator protein: Mechanistic insight into ganglioside GM2 degradation
Michaela Wendeler,Joerg Hoernschemeyer,Daniel Hoffmann,Thomas Kolter,Guenter Schwarzmann,Konrad Sandhoff +5 more
TL;DR: Results provide strong evidence that the hydrophobicity and the great structural mobility of this element are crucial for the extraction of the membrane-embedded glycolipid, its stabilization inside the spacious cavity and its guidance to the enzyme's active site.
Journal ArticleDOI
Expression of recombinant human GM2-activator protein in insect cells: purification and characterization by mass spectrometry.
Michaela Wendeler,Thorsten Lemm,Judith Weisgerber,Joerg Hoernschemeyer,Oliver Bartelsen,Ute Schepers,Konrad Sandhoff +6 more
TL;DR: The biological activity of the recombinant protein was demonstrated by its ability to stimulate the hexosaminidase A-catalyzed degradation of ganglioside GM2, and the homogeneity and glycosylation were assessed by ESI-TOF mass spectrometry.
Journal ArticleDOI
Expression of the GM2-activator protein in the methylotrophic yeast Pichia pastoris, purification, isotopic labeling, and biophysical characterization.
Michaela Wendeler,Joerg Hoernschemeyer,Michael John,Norbert Werth,Maike Schoeniger,Thorsten Lemm,Rudolf Hartmann,Horst Kessler,Konrad Sandhoff +8 more
TL;DR: A convenient eukaryotic system that allows the efficient production of functionally folded, glycosylated GM2AP and offers the potential of cost-efficient isotopic labeling for structural studies by NMR spectroscopy is established.