Johan de Rooij

TL;DR: A novel cAMP analogue is developed, 8CPT-2Me-cAMP, which activates Epac, but not PKA, both in vitro and in vivo, and tests the widespread model that Rap1 mediates cAMP-induced regulation of the extracellular signal-regulated kinase (ERK).

TL;DR: In this paper, the relative role of cAMP-dependent protein kinase (cAPK) and guanine exchange factor directly activated by cAMP (Epac) was investigated.

TL;DR: It is demonstrated that external stimuli of cAMP signaling, i.e., isoproterenol, which activates the Gαs-coupled β2-adrenergic receptor can induce integrin-mediated cell adhesion through the Epac-Rap1 pathway.

TL;DR: Bead-twisting measurements andplementary investigations of cadherin-based rigidity sensing suggest that, although &agr;-catenin alters traction force generation, it is not the sole regulator of cell contractility on compliant cadhersin-coated substrata.

TL;DR: It is concluded that, in vivo, p85 and Ras synergize to activate PI 3-kinase and that strong activation of only endogenous Ras exerts a small effect on PI 3 -kinase activity, sufficient for PKB activation but not lamellipodium formation.