J
John F. Engelhardt
Researcher at Roy J. and Lucille A. Carver College of Medicine
Publications - 371
Citations - 28881
John F. Engelhardt is an academic researcher from Roy J. and Lucille A. Carver College of Medicine. The author has contributed to research in topics: Cystic fibrosis & Cystic fibrosis transmembrane conductance regulator. The author has an hindex of 88, co-authored 340 publications receiving 26574 citations. Previous affiliations of John F. Engelhardt include University of Iowa & Johns Hopkins University School of Medicine.
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Journal ArticleDOI
A revised airway epithelial hierarchy includes CFTR-expressing ionocytes
Daniel T. Montoro,Adam L. Haber,Moshe Biton,Moshe Biton,Vladimir Vinarsky,Brian M. Lin,Susan E. Birket,Feng Yuan,Sijia Chen,Hui Min Leung,Jorge Villoria,Noga Rogel,Grace Burgin,Alexander M. Tsankov,Avinash Waghray,Michal Slyper,Julia Waldman,Lan Nguyen,Danielle Dionne,Orit Rozenblatt-Rosen,Purushothama Rao Tata,Hongmei Mou,Manjunatha Shivaraju,Hermann Bihler,Martin Mense,Guillermo J. Tearney,Steven M. Rowe,John F. Engelhardt,Aviv Regev,Aviv Regev,Jayaraj Rajagopal +30 more
TL;DR: ‘pulse-seq’ is developed, combining scRNA-seq and lineage tracing, to show that tuft, neuroendocrine and ionocyte cells are continually and directly replenished by basal progenitor cells, establishing a new cellular narrative for airways disease.
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Ablation of E2A in recombinant adenoviruses improves transgene persistence and decreases inflammatory response in mouse liver
TL;DR: Second-generation recombinant adenoviruses harboring a beta-galactosidase-expressing transgene in which a temperature-sensitive mutation has been introduced into the E1-deleted recombinant are described.
Journal ArticleDOI
Submucosal glands are the predominant site of CFTR expression in the human bronchus.
John F. Engelhardt,James R. Yankaskas,Stephen A. Ernst,Yiping Yang,Christopher R. Marino,Richard C. Boucher,Jonathan A. Cohn,James M. Wilson +7 more
TL;DR: The data suggest mechanisms whereby defects in CFTR expression could lead to abnormal production of mucus in human lung, particularly in non–CF individuals.
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Successful ex vivo gene therapy directed to liver in a patient with familial hypercholesterolaemia
Mariann Grossman,Steven E. Raper,Steven E. Raper,Karen Kozarsky,Karen Kozarsky,Evan A. Stein,John F. Engelhardt,John F. Engelhardt,David W.M. Muller,Paul J. Lupien,James M. Wilson,James M. Wilson +11 more
TL;DR: The treatment of a 29 year old woman with homozygous FH by ex vivo gene therapy directed to liver represents the first report of human gene therapy in which stable correction of a therapeutic endpoint has been achieved.
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A controlled study of adenoviral-vector-mediated gene transfer in the nasal epithelium of patients with cystic fibrosis
Michael R. Knowles,Kathy Hohneker,Zhaoqing Zhou,John C. Olsen,Terry L. Noah,Ping Chuan hu,Margaret W. Leigh,John F. Engelhardt,Lloyd J. Edwards,Kim R. Jones,Mariann Grossman,James M. Wilson,Larry G. Johnson,Richard C. Boucher +13 more
TL;DR: In patients with cystic fibrosis, adenoviral-vector-mediated transfer of the CFTR gene did not correct functional defects in nasal epithelium, and local inflammatory responses limited the dose of adenvirus that could be administered to overcome the inefficiency of gene transfer.