J
John S. Coukos
Researcher at University of Chicago
Publications - 11
Citations - 272
John S. Coukos is an academic researcher from University of Chicago. The author has contributed to research in topics: Medicine & Methylglyoxal. The author has an hindex of 4, co-authored 8 publications receiving 159 citations.
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Journal ArticleDOI
A metabolite-derived protein modification integrates glycolysis with KEAP1–NRF2 signalling
Michael J. Bollong,Gihoon Lee,John S. Coukos,Hwayoung Yun,Hwayoung Yun,Claudio Zambaldo,Jae Won Chang,Emily N. Chin,Insha Ahmad,Arnab Chatterjee,Luke L. Lairson,Peter G. Schultz,Raymond E. Moellering +12 more
TL;DR: The existence of direct inter-pathway communication between glycolysis and the KEAP1–NRF2 transcriptional axis is demonstrated, insight is provided into the metabolic regulation of the cellular stress response, and a therapeutic strategy is suggested for controlling the cytoprotective antioxidant response in several human diseases.
Journal ArticleDOI
Synthetic antibodies against BRIL as universal fiducial marks for single−particle cryoEM structure determination of membrane proteins
Somnath Mukherjee,Satchal K. Erramilli,Mark Ammirati,Frances J. D. Alvarez,Kimberly F. Fennell,Michael D. Purdy,Blazej M. Skrobek,Blazej M. Skrobek,Katarzyna Radziwon,Katarzyna Radziwon,John S. Coukos,Yanyong Kang,Yanyong Kang,Przemysław Dutka,Przemysław Dutka,Xiang Gao,Xiayang Qiu,Mark Yeager,H. Eric Xu,H. Eric Xu,Seungil Han,Anthony A. Kossiakoff +21 more
TL;DR: This work generates recombinant antibodies against the 12 kDa fusion partner BRIL domain from apocytochrome b562 to use them as plug and play fiducial marks for structure determination of BRIL fused membrane proteins.
Journal ArticleDOI
Versatile Peptide Macrocyclization with Diels-Alder Cycloadditions.
Jeffrey E. Montgomery,Justin A. Donnelly,Sean W. Fanning,Thomas E. Speltz,Xianghang Shangguan,John S. Coukos,Geoffrey L. Greene,Raymond E. Moellering +7 more
TL;DR: It is demonstrated that Diels-Alder [4+2] cy-cloadditions can be harnessed for peptide macrocyclization and stabilization within a range of peptide scaffolds and chemical environ-ments.
Journal ArticleDOI
Profiling Reactive Metabolites via Chemical Trapping and Targeted Mass Spectrometry.
TL;DR: This work takes advantage of the reactive acylphosphate group in 1,3-BPG to chemically trap the metabolite with hydroxylamine during metabolite isolation, enabling quantitative analysis by targeted LC-MS/MS.
Journal ArticleDOI
Interaction profiling methods to map protein and pathway targets of bioactive ligands.
TL;DR: Several emerging proteomic technologies capable of detecting protein interaction networks in live cells and their integration to draft holistic maps of proteins that respond to diverse stimuli, including bioactive small molecules are reviewed.