Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction

Coffee Pulp: An Industrial By-product with Uses in Agriculture, Nutrition and Biotechnology

Caffeine: A potential strategy to improve survival of neonatal pigs and sheep.

Aim: The purpose of this study was to seek the acute effects of coffee intake on glucose metabolism and some hormones
Materials and Methods: The participants were asked to drink a cup of instant coffee (2 g) Three hours later, the glucose, insulin, glucagon, leptin, cortisol, total cholesterol, triglyceride, HDL, LDL, CRP, TSH and free T4 tests were repeated The participants did not consume any other drink or food between 0 and 3 h The participants were called back one week later In 0 h, the glucose, insulin, glucagon, leptin, cortisol, total cholesterol, triglyceride, HDL, LDL, CRP, TSH and free T4 tests were performed This time, the participants were asked to drink a glass of hot water Three hours later, same tests were repeated
Results: There weren't any significant changes in the levels of insulin, glucagon, cortisol, lipid levels, CRP, TSH and free T4 tests after coffee intake but we found significant changes in the leptin and plasma glucose values
Conclusions: In conclusion, acute coffee intake does not have an acute impact on glucagon, cortisol, and TSH levels but affects leptin levels which can be one of the mechanisms of type diabetes mellitus reduction with coffee intake

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The acute effect of coffee intake on hormones that affect glucose and glucose metabolism in healthy individuals

Se usó un total de 172 ovejas multíparas con peso corporal promedio de 59 kg distribuidas aleatoriamente en cuatro tratamientos: CIDR1 con dispositivos nuevos (tratamiento testigo) y reutilizados CIDR2, CIDR3, CIDR4 (con 6, 12 y 18 días de uso previo, respectivamente). Las variables inicio de estro e índice de prolificidad se analizaron con la prueba de Kruskal Wallis; el inicio de estro, en promedio se presentó más tarde en el tratamiento con CIDR nuevos (40±10 h) (P≤0.05) respecto a los de primera reutilización (31±9 h), no hubo diferencia con los de segunda y tercera reutilización. La presencia de estro, porcentaje de gestación, porcentaje de fertilidad y tipo de parto se evaluaron por medio de la prueba de chi-cuadrada y no hubo diferencias (P>0.05) entre tratamientos. La presencia de estro osciló entre 93 y 100%, el valor promedio de gestación fue 80.8%, 80.2% para la porcentaje de fertilidad y 1.26 para el índice de prolificidad. El porcentaje de partos sencillos (71, 72, 79, y 57% en CIDR1, CIDR2, CIDR3, y CIDR4, respectivamente) fueron mayores (P≤0.05) a los partos dobles. Las mediciones de concentraciones de progesterona en suero sanguíneo se realizaron mediante la técnica de ELISA, en donde se observó diferencia (P>0.05) entre el tratamiento de cuarto uso de CIDR respecto a los demás tratamientos. Se concluye que el uso de CIDR en protocolos de 6 días permite que los dispositivos sean utilizados hasta por cuarta ocasión.

Sincronización de estros en ovejas multíparas con CIDR de primera, segunda y tercera reutilización

The objective of the present research was to determine the effect of long synchronization protocols based on reused progesterone devices (controlled internal drug release [CIDR]) associated with different doses of equine chorionic gonadotropin (eCG) on reproductive variables in crossbred sheep (Suffolk × Kathadin × Dorset). The CIDR were used for eleven days in a previous study in sheep from the same herd and were washed and disinfected before reusing. Sixty-four sheep, in the reproductive season, were randomly assigned to four experimental groups (n = 16). Treatments consisted of a group with 10 d CIDR and 300 IU eCG; a group with 10 d CIDR and 400 IU of eCG; a group with 12 d CIDR and 300 IU of eCG; and a group with 12 d CIDR and 400 IU of eCG. A completely randomized design was used. There was an estrous presentation rate of 100% in all treatments. The begining of estrous, gestation rate, fertility rate, type of parturition, and prolificacy index were equal between groups. Progesterone serum concentration was higher in sheep from the 10 d CIDR groups. The CIDR, reused for the second time, associated with 300 or 400 IU of eCG for estrous synchronization in sheep, are effective to obtain good pregnancy rates and ensures higher prolificacy rates.

https://www.rbz.org.br/wp-content/uploads/articles_xml/1806-9290-rbz-50-e20200176/1806-9290-rbz-50-e20200176.pdf

Estrous synchronization in sheep with reused progesterone devices and eCG

Background: Coffee pulp has a high content of antioxidants capable of modifying the oxidative status in small ruminants. However, high amounts for a prolonged time can reduce fertility. Objective: To determine the effect of two inclusion levels of coffee pulp during estrous synchronization on reproductive variables and oxidative status of primiparous ewes. Methods: Sixty Suffolk x Dorset primiparous ewes were distributed into three treatments in a completely randomized design; T0: (n=20) 1.5 kg balanced diet, Tl: (n=20) 1.5 kg balanced diet and 5% coffee pulp, T2: (n=20) 1.5 kg balanced diet and 10% coffee pulp. Supplementation was given for 16 days before estrus synchronization and until the beginning of the breeding season. A progestogen (CIDR®) was inserted for ll days and a dose of PGF2a was applied two days prior to its withdrawal. Estrus detection started 12 hours after CIDR withdrawal. Blood samples were obtained during the supplementation period to measure oxidative status, antioxidant capacity, glucose and insulin, and up to 9 days after breeding to determine progesterone concentration. Pregnancy diagnosis was performed at 30 and 60 days post-breeding. An analysis of repeated measures of mixed effects and frequency analysis were carried out. Results: Inclusion of coffee pulp for a short period prior to breeding did not affect reproductive parameters, nor progesterone, glucose or insulin concentrations (p>0.05); however, antioxidant capacity increased, while lipid oxidation showed an opposite trend (p<0.05). Conclusion: Inclusion of up to 10% coffee pulp in the diet of ewe lambs for 16 days prior to breeding improves oxidative status without causing adverse effects on pregnancy, estrus or prolificacy.

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Coffee pulp supplementation prior to breeding improves oxidative status without affecting fertility of primiparous ewes

Objective: To evaluate the effect of different doses of N, P, K fertilizers and two biostimulants, on growth parameters and vegetative development in plants of the species Z. lindleyana Herb.
Design/methodology/approach: An experiment was established under greenhouse conditions under a CRD; ten treatments with five repetitions were tested, with different doses of N, P and K and two biostimulants. The experimental unit was one plant per pot. Data were analyzed with descriptive statistics, non-parametric Kruskal-Wallis tests (α=0.05). Budding, flowering, leaf height, number of leaves and seed production were measured.
Results: Vegetative development was distinguished in all plants. Only six treatments showed flowering. The percentage of floral and vegetative sprouting did not show delay or advance in the physiology of the plant. In leaf height and number of leaves, different behaviors were detected in the treatments, not detected in the Kruskal-Wallis tests. The plants treated with high doses of NPK fertilization and combined with biostimulants showed inflorescences and seed production.
Limitations on study/implications: Being a native plant with ornamental potential, the collection of this species is a challenge due to the lack of studies on the potential distribution of the species.
Findings/conclusions: This species can be cultivated under a greenhouse and pot planting system. Floral budding, height and number of leaves is stimulated by high doses of nitrogen. The combination of NPK and biostimulants favors development/growth and seed production.

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José Luis García-Cué

Papers

Estrous synchronization in sheep with reused progesterone devices and eCG

Coffee pulp supplementation prior to breeding improves oxidative status without affecting fertility of primiparous ewes

Evaluation of the application of fertilizers and biostimulants in Zephyranthes lindleyana Herb (Amarylidaceae) under greenhouse conditions