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Sergey Shmakov
Researcher at National Institutes of Health
Publications - 45
Citations - 7045
Sergey Shmakov is an academic researcher from National Institutes of Health. The author has contributed to research in topics: CRISPR & Genome. The author has an hindex of 19, co-authored 41 publications receiving 4760 citations. Previous affiliations of Sergey Shmakov include Rutgers University & Skolkovo Institute of Science and Technology.
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Journal ArticleDOI
C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector
Omar O. Abudayyeh,Jonathan S. Gootenberg,Silvana Konermann,Silvana Konermann,Silvana Konermann,Julia Joung,Julia Joung,Julia Joung,Ian Slaymaker,Ian Slaymaker,Ian Slaymaker,David Benjamin Turitz Cox,Sergey Shmakov,Sergey Shmakov,Kira S. Makarova,Ekaterina Semenova,Leonid Minakhin,Konstantin Severinov,Konstantin Severinov,Konstantin Severinov,Aviv Regev,Aviv Regev,Eric S. Lander,Eric S. Lander,Eric S. Lander,Eugene V. Koonin,Feng Zhang +26 more
TL;DR: LshC2c2 is a RNA-guided RNase which requires the activity of its two HEPN domains, suggesting previously unidentified mechanisms of RNA targeting and degradation by CRISPR systems.
Journal ArticleDOI
Evolutionary classification of CRISPR-Cas systems: a burst of class 2 and derived variants.
Kira S. Makarova,Yuri I. Wolf,Jaime Iranzo,Sergey Shmakov,Omer S. Alkhnbashi,Stan J. J. Brouns,Emmanuelle Charpentier,David R. Cheng,Daniel H. Haft,Philippe Horvath,Sylvain Moineau,Francisco J. M. Mojica,David Scott,Shiraz A. Shah,Virginijus Siksnys,Michael P. Terns,Česlovas Venclovas,Malcolm F. White,Alexander F. Yakunin,Alexander F. Yakunin,Winston X. Yan,Feng Zhang,Roger A. Garrett,Rolf Backofen,John van der Oost,Rodolphe Barrangou,Eugene V. Koonin +26 more
TL;DR: An updated evolutionary classification of CRISPR–Cas systems and cas genes is provided, with an emphasis on the major developments that have occurred since the publication of the latest classification, in 2015, which includes 2 classes, 6 types and 33 subtypes.
Journal ArticleDOI
Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems
Sergey Shmakov,Omar O. Abudayyeh,Kira S. Makarova,Yuri I. Wolf,Jonathan S. Gootenberg,Ekaterina Semenova,Leonid Minakhin,Julia Joung,Silvana Konermann,Konstantin Severinov,Konstantin Severinov,Konstantin Severinov,Feng Zhang,Eugene V. Koonin +13 more
TL;DR: In this article, three distinct Class 2 CRISPR-Cas systems are described, two of which contain RuvC-like endonuclease domains distantly related to Cpf1.
Journal ArticleDOI
Diversity and evolution of class 2 CRISPR-Cas systems
Sergey Shmakov,Aaron Smargon,David A. Scott,David Benjamin Turitz Cox,Neena Pyzocha,Winston X. Yan,Omar O. Abudayyeh,Jonathan S. Gootenberg,Kira S. Makarova,Yuri I. Wolf,Konstantin Severinov,Konstantin Severinov,Konstantin Severinov,Feng Zhang,Eugene V. Koonin +14 more
TL;DR: A comprehensive census of class 2 types and class 2 subtypes in complete and draft bacterial and archaeal genomes is presented, evolutionary scenarios for the independent origin of different class 2 CRISPR–Cas systems from mobile genetic elements are outlined, and an amended classification and nomenclature of CRISpr–Cas is proposed.
Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems
Sergey Shmakov,Omar O. Abudayyeh,Kira S. Makarova,Yuri I. Wolf,Jonathan S. Gootenberg,Ekaterina Semenova,Leonid Minakhin,Julia Joung,Silvana Konermann,Konstantin Severinov,Konstantin Severinov,Konstantin Severinov,Feng Zhang,Eugene V. Koonin +13 more
TL;DR: Comparison analysis indicates that Class 2 CRISPR-Cas systems evolved on multiple occasions through recombination of Class 1 adaptation modules with effector proteins acquired from distinct mobile elements.