Kazuhiro Iwashita

TL;DR: Specific expansion of genes for secretory hydrolytic enzymes, amino acid metabolism and amino acid/sugar uptake transporters supports the idea that A. oryzae is an ideal microorganism for fermentation.

TL;DR: A comparative proteome analysis of extracellular proteins in solid-state and submerged (liquid) cultures suggested that the secretion of TAA and Bgl was regulated by trapping these proteins in the cell wall in submerged culture and that secretion of GlaA and XynG2 was regulated at the posttranscriptional level in the solid- state culture.

TL;DR: The draft whole-genome shotgun sequence of a commonly used diploid sake yeast strain, Kyokai no. 7 (K7), is reported, finding that two regions in chromosomes I and VII of S288C have apparently been replaced by Ty elements in K7.

TL;DR: Solid-state specific gene expression was observed in the glaB and pepA genes of Aspergillus oryzae and ESP and the cell wall fraction of A. kawachii were shown to be involved in the stability of beta-glucosidases, revealing the new aspects of protein production in filamentous fungi.

TL;DR: From the results of N-terminal amino acid sequence, enzymatic parameters and deglycosylation of the three purified enzymes, it is strongly suggested that these three enzymes were products of the same gene, modified by different degree of glycosylated.