K
Kees L. M. C. Franken
Researcher at Leiden University Medical Center
Publications - 154
Citations - 8345
Kees L. M. C. Franken is an academic researcher from Leiden University Medical Center. The author has contributed to research in topics: Antigen & Mycobacterium tuberculosis. The author has an hindex of 51, co-authored 142 publications receiving 7669 citations. Previous affiliations of Kees L. M. C. Franken include Leiden University.
Papers
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Journal ArticleDOI
Blockade of the granzyme B/perforin pathway through overexpression of the serine protease inhibitor PI-9/SPI-6 constitutes a mechanism for immune escape by tumors
Jan Paul Medema,J. de Jong,L. T. C. Peltenburg,E. M. E. Verdegaal,Arko Gorter,S. A. Bres,Kees L. M. C. Franken,Michael Hahne,J. P. Albar,C. J. M. Melief,R. Offringa +10 more
TL;DR: The data reveal that PI-9/SPI-6 is an important parameter determining the success of T cell-based immunotherapeutic modalities against cancer, and expression results in the resistance of tumor cells to CTL-mediated killing both in vitro and in vivo.
Journal ArticleDOI
Human T-cell responses to 25 novel antigens encoded by genes of the dormancy regulon of Mycobacterium tuberculosis.
Eliane M. S. Leyten,May Young Lin,Kees L. M. C. Franken,Annemieke H. Friggen,Corine Prins,Krista E. van Meijgaarden,Martin I. Voskuil,Karin Weldingh,Peter Andersen,Gary K. Schoolnik,Sandra M. Arend,Tom H. M. Ottenhoff,Michèl R. Klein +12 more
TL;DR: Especially strong IFN-gamma responses to latency antigens were observed in latently infected individuals, suggesting that immune responses against these antIGens may contribute to controlling latent M. tuberculosis infection.
Journal ArticleDOI
Purification of his-tagged proteins by immobilized chelate affinity chromatography: the benefits from the use of organic solvent.
Kees L. M. C. Franken,Hoebert S. Hiemstra,Krista E. van Meijgaarden,Yanri W. Subronto,J. den Hartigh,Tom H. M. Ottenhoff,Jan W. Drijfhout +6 more
TL;DR: A simple and fast, one-step protocol to remove impurities highly efficiently from E. coli proteins, endotoxins, and detergents is described and it is shown that proteins are able to refold correctly after isopropanol treatment.
Journal ArticleDOI
CD8+ CTL Priming by Exact Peptide Epitopes in Incomplete Freund’s Adjuvant Induces a Vanishing CTL Response, whereas Long Peptides Induce Sustained CTL Reactivity
Martijn S. Bijker,Susan J. F. van den Eeden,Kees L. M. C. Franken,Cornelis J. M. Melief,Rienk Offringa,Sjoerd H. van der Burg +5 more
TL;DR: The data clearly show why priming of CTL with minimal peptide epitopes in IFA is suboptimal, and demonstrate that the use of longer versions of these CTL peptides ensures the induction of sustained effector CD8+ T cell reactivity in vivo.
Journal ArticleDOI
Success or failure of vaccination for HPV16-positive vulvar lesions correlates with kinetics and phenotype of induced T-cell responses
Marij J. P. Welters,Gemma G. Kenter,Peggy J. de Vos van Steenwijk,Margriet J. G. Löwik,Dorien M A Berends-van der Meer,Farah Essahsah,Linda F. M. Stynenbosch,Annelies P G Vloon,Tamara H. Ramwadhdoebe,Sytse J. Piersma,Jeanette M. van der Hulst,A. Rob P. M. Valentijn,Lorraine M. Fathers,Jan W. Drijfhout,Kees L. M. C. Franken,Jaap Oostendorp,Gert Jan Fleuren,Cornelis J. M. Melief,Sjoerd H. van der Burg +18 more
TL;DR: It is demonstrated that the vaccine-prompted level of this population of patients with human papillomavirus type 16 (HPV16)-induced vulvar intraepithelial neoplasia grade 3 is associated with early treatment failure.