Kiyoshi Mizuuchi

TL;DR: Observations account for the role of endonuclease VII in the DNA metabolism of phage T4, and provide the first example of an enzyme that acts specifically on branch points in duplex DNA.

TL;DR: It is suggested that the intracellular formation of large cruciform structures is incompatible with survival of the DNA species Escherichia coli.

TL;DR: The Mu A protein protection patterns on the L ends of Mu and the closely related phage D108 are essentially identical and it is shown that the A proteins ofMu and D108 can function, at different efficiencies, interchangeably on the Mu and D 108 L ends in vivo.

TL;DR: The transposition-replication reaction of phage Mu has been reproduced in a cell-free reaction system and both types of products show incorporation of radioactive DNA precursors; however, simple inserts do not seem to undergo a full round of DNA replication.

TL;DR: The discovery and characterization of a new intermediate in Mu DNA transposition allowed assembly of the transposition machinery to be separated from the chemical steps of recombination and the participation of internal sequence elements and accessory factors exclusively during SSC assembly allows recombination to be controlled prior to the irreversible chemical steps.