Journal ArticleDOI
T4 endonuclease VII cleaves holliday structures
TLDR
Observations account for the role of endonuclease VII in the DNA metabolism of phage T4, and provide the first example of an enzyme that acts specifically on branch points in duplex DNA.About:
This article is published in Cell.The article was published on 1982-06-01. It has received 259 citations till now. The article focuses on the topics: Branch migration & DNA ligase.read more
Citations
More filters
Journal ArticleDOI
Specific recognition of cruciform DNA by nuclear protein HMG1.
TL;DR: Biochemical and immunological data showed that this protein is HMG1, an evolutionarily conserved, essential, and abundant component of the nucleus, which points to a critical role for cruciform DNA conformations.
Journal ArticleDOI
A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers
Jeff Elhai,C P Wolk +1 more
TL;DR: A general method is described to convert virtually any small or medium-sized plasmid into a positive-selection vector and several symmetrical polylinkers and drug-resistance cassettes within the vectors.
Journal ArticleDOI
Processing of recombination intermediates by the RuvABC proteins.
TL;DR: Genetic and biochemical studies indicate that branch migration and resolution are coupled by direct interactions between the three proteins, possibly by the formation of a RuvABC complex.
Journal ArticleDOI
DNA Packaging in dsDNA Bacteriophages
TL;DR: The present work investigated the mechanism and mechanism of ATP-driven DNA translocation into the prohead and the structure of packaged phage DNA, as well as the interactions of DNA packaging with other DNA processes in vivo.
References
More filters
Journal ArticleDOI
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing
TL;DR: An approach to DNA sequencing using chain-terminating inhibitors (Sanger et al., 1977) combined with cloning of small fragments of DNA in a single-stranded DNA bacteriophage is described, determining the 2771-nucleotide sequence of the largest MboI restriction enzyme fragment from human mitochondrial DNA.
Book ChapterDOI
[31] Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids
Journal ArticleDOI
A mechanism for gene conversion in fungi.
TL;DR: The model indicates how precise breakage and rejoining of chromatids could occur in the vicinity of the conversion, so that conversion would frequently be accompanied by the recombination of outside markers.
Journal ArticleDOI
Cruciform structures in supercoiled DNA
TL;DR: Specific sites in supercoiled pVH51 and pBR322 DNAs are cleaved by single-strand specific nucleases in the loops of potential cruciform structures.
Journal ArticleDOI
Mapping of Deletions and Substitutions in Heteroduplex DNA Molecules of Bacteriophage Lambda by Electron Microscopy
TL;DR: Electron microscopy of heteroduplex DNA molecules, composed of one strand of Escherichia coli phage λ+ DNA annealed to the complementary DNA strand of a λ deletion or substitution mutant, permits visualization, as well as precise measurements and mapping, of the unpaired single-stranded regions of nonhomology in the otherwise double- Stranded molecules.